Fig. 2
From: Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD

Standard curve of 8-OHGua obtained from the 8-OHGua standard in the diluent (1.8% acetonitrile, 62 mM NaOAc (pH 4.5) and 0.01 mM H2SO4) (open circles) and curve of the 8-OHGua-spiked saliva (closed circles; the original 8-OHGua peak level of an authentic saliva sample (20.64) was subtracted)