Table 1 Characteristic comparison among the Saccharomyces cerevisiae Grxs proteins

Number of amino acids

110

143

250

244

150

231

203

109

Formula

C₅₄₉H₈₈₆N₁₄₆O₁₇₀S₄

C₇₁₅H₁₁₆₁N₁₈₁O₂₁₃S₅

C₁₂₅₀H₁₉₂₂N₃₂₂O₄₁₁S₇

C₁₂₂₆H₁₉₁₀N₃₁₆O₃₈₉S₆

C₇₆₂H₁₂₀₀N₁₉₆O₂₂₇S₆

C₁₁₃₉H₁₈₃₄N₃₀₆O₃₆₁S₆

C₉₉₁H₁₅₆₄N₂₇₄O₃₂₀S₄

C₅₇₁H₈₇₉N₁₄₉O₁₆₀S₄

Molecular weight(Da)

12,380.19

15,861.47

28,261.34

27,492.90

16,931.45

25,783.28

22,565.20

12,519.40

pI

4.98

6.73

4.37

4.57

4.85

6.01

5.64

7.78

Instability index

40.59

32.24

51.79

46.11

60.33

35.77

41.82

35.73

Aliphatic index

107.27

104.97

81.12

83.93

86.53

89.87

81.58

83.12

Grand average of hydropathicity (GRAVY)

−0.248

0.024

−0.470

−0.355

−0.259

−0.412

−0.437

−0.260

Number of cysteines

2

2

3

2

2

1

1

2

PDB ID

3C1R 3C1S

3CTF 3CTG

5Y4U 5Y4T

3GX8

5J3R 3L4N

2 M80

Ligands

MES GSH

SO4

GSH + Fe2 S2 GSH

location

Cytosolic

Major one at the cytosol and a minor one at mitochondria

Nucleus

Nucleus

Mitochondrial matrix

Vacuole

Membrane

Cytoplasm

Active site

CPYC

CPYC

CGFS

CGFS

CSYS

CSYS

CPYS

CPDS

Function

In defence against oxidative stress

In defence against oxidative stress

Regulate the activity of transcription factors such as Aft1

Regulate the activity of transcription factors such as Aft1

Participates in the synthesis of iron–sulfur clusters.

Not an essential gene

Iron-Sulfur Cluster Binding

No

No

Yes

Yes

Yes

Yes

No

No

HEDS assay

Yes

Yes

No

No

No

Yes

Yes

Very weak