Genes and Environment

Table 2 Mutation frequency in the lacZ and cII genes from the nasal tissues of Muta™ mice treated with 2,5-, 2,6- and 3,5-DMAs

From: Mutagenic properties of dimethylaniline isomers in mice as evaluated by comet, micronucleus and transgenic mutation assays

Animal ID		lacZ				cII
Animal ID		Total plaques^a	Mutants	MF (× 10⁶)	Mean ± SD	Total plaques^a	Mutants	MF (× 10⁶)	Mean ± SD
Control (olive oil)	41	(36000)	5	138.9		(59400)	4	67.3
	42	(13000)	2	153.8		(10800)	1	92.6
	43	373,000	19	50.9		399,600	10	25.0
	44	306,000	9	29.4		321,000	5	15.6
	45	477,500	14	29.3		396,600	7	17.7
	Total	1,205,500	49	40.6	36.6 ± 10.2	1,187,400	27	22.7	19.4 ± 4.1
2,5-DMA	81	471,000	40	84.9		546,000	41	75.1
	82	821,000	71	86.5		871,200	40	45.9
	83	388,500	42	108.1		639,600	28	43.8
	84	731,000	70	95.8		916,800	32	34.9
	85	566,500	40	70.6		552,600	31	56.1
	Total	2,978,000	263	88.3	89.2 ± 12.4 ^c	3,526,200	172	48.8	51.2 ± 13.7 ^b
2,6-DMA	91	172,500	9	52.2		261,000	6	23.0
	92	260,000	26	100.0		429,600	13	30.3
	93	391,000	26	66.5		493,200	46	93.3
	94	(0)	0	0		(0)	0	0
	95	252,000	29	115.1		379,200	21	55.4
	Total	1,075,500	90	83.7	83.4 ± 25.2 ^b	1,563,000	86	55.0	50.5 ± 27.5
3,5-DMA	101	276,500	11	39.8		1,854,600	16	8.6
	102	554,500	49	88.4		850,800	27	31.7
	103	665,500	23	34.6		810,000	18	22.2
	104	601,500	22	36.6		411,600	22	53.4
	105	192,000	14	72.9		246,000	11	44.7
	Total	2,290,000	119	52.0	54.4 ± 22	4,173,000	94	22.5	32.1 ± 15.9

^aData with at least 100,000 total plaques were evaluated by the statistical analysis and a parenthesis indicates exclusion
^bSignificantly different from the control (P < 0.05) by t-test
^cSignificantly different from the control (P < 0.01) by t-test

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