Table 4 Mutation frequency in the lacZ and cII genes from the bone marrow of Muta™ mice treated with 2,5-, 2,6- and 3,5-DMAs
Animal ID | lacZ | cII | |||||||
|---|---|---|---|---|---|---|---|---|---|
Total plaquesa | Mutants | MF (×106) | Mean ± SD | Total plaquesa | Mutants | MF (×106) | Mean ± SD | ||
Control (olive oil) | 41 | 123,000 | 4 | 32.5 | 125,400 | 1 | 8.0 | ||
42 | (88500) | 5 | 56.5 | 227,400 | 4 | 17.6 | |||
43 | (33000) | 0 | 0 | 326,100 | 12 | 36.8 | |||
44 | (32500) | 0 | 0 | (37500) | 1 | 26.7 | |||
45 | (60500) | 2 | 33.1 | (87000) | 2 | 23.0 | |||
Total | 337,500 | 11 | 32.6 | 32.5 | 803,400 | 20 | 24.9 | 20.8 ± 12.0 | |
2,5-DMA | 81 | (42000) | 7 | 166.7 | 122,700 | 2 | 16.3 | ||
82 | 320,500 | 26 | 81.1 | 422,400 | 6 | 14.2 | |||
83 | 238,500 | 17 | 71.3 | 374,400 | 19 | 50.7 | |||
84 | 215,000 | 5 | 23.3 | 162,000 | 8 | 49.4 | |||
85 | (71500) | 9 | 125.9 | 105,300 | 3 | 28.5 | |||
Total | 887,500 | 64 | 72.1 | 58.6 ± 25.3 | 1,186,800 | 38 | 32.0 | 31.8 ± 15.7 | |
2,6-DMA | 91 | 155,000 | 12 | 77.4 | 272,100 | 3 | 11.0 | ||
92 | 120,000 | 17 | 141.7 | 216,300 | 14 | 64.7 | |||
93 | 676,000 | 12 | 17.8 | 240,300 | 4 | 16.6 | |||
94 | (95500) | 1 | 10.5 | (91200) | 3 | 32.9 | |||
95 | 584,500 | 31 | 53.0 | 509,700 | 24 | 47.1 | |||
Total | 1,631,000 | 73 | 44.8 | 72.5 ± 45.2 | 1,329,600 | 48 | 36.1 | 34.9 ± 22.0 | |
3,5-DMA | 101 | 793,500 | 13 | 16.4 | 392,700 | 13 | 33.1 | ||
102 | 250,000 | 12 | 48.0 | 263,400 | 7 | 26.6 | |||
103 | (55000) | 4 | 72.7 | 121,800 | 6 | 49.3 | |||
104 | (70000) | 2 | 28.6 | (95700) | 2 | 20.9 | |||
105 | 119,500 | 5 | 41.8 | 140,100 | 4 | 28.6 | |||
Total | 1,288,000 | 36 | 28.0 | 35.4 ± 13.7 | 1,013,700 | 32 | 31.6 | 34.4 ± 8.9 | |