Skip to main content
Fig. 3 | Genes and Environment

Fig. 3

From: High-content imaging analyses of γH2AX-foci and micronuclei in TK6 cells elucidated genotoxicity of chemicals and their clastogenic/aneugenic mode of action

Fig. 3

Representative images captured with an imaging cytometer of TK6 cells. Images other than C were obtained at the same time, from the same well. (A1) TK6 cells stained with Hoechst33258 for nuclei and CellMask Red for cytoplasm. The original monochrome images were given pseudo colors, yellow for nuclei and red for cytoplasm. (A2) The arrow shows a MN detected by automatic imaging analysis. (B1) γH2AX foci stained immunocytometrically with AlexaFluor 488-labeled antibody. (B2) Foci of γH2AX (FH) were detected by imaging-analysis. The γH2AX foci and MNi were simultaneously detected by the common protocol of imaging analysis. (C) Pan-nuclear staining of γH2AX (PH), which would be induced in apoptotic cells, was excluded from the analyses

Back to article page

Genes and Environment

ISSN: 1880-7062

Contact us