Table 1 The polymerase Chain Reaction (PCR) conditions
Method | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | PCR reaction conditions | ||
|---|---|---|---|---|---|
McCauley et al. [25] | CTGGAGACCACTCCCATCCTTTCT | GATGTGGCCATCACATTCGTCAGAT | Denaturation 95 °C for 1 min | Annealing 30 cycles of | Extension 72 °C for 10 min |
|  | 95 °C for 1 min |  | |||
|  | 58 °C for 1 min |  | |||
|  | 72 °C for 2 min |  | |||