Fig. 6
From: Development of a novel PIG-A gene mutation assay based on a GPI-anchored fluorescent protein sensor
Detection of mutations after MMS treatment. a GPI(−) phenotypic expression time for TK6 cells. Cells were treated with 0, 12 or 24 μM MMS for 24 h, washed and cultured in fresh medium. GPI(−) frequency was detected by flow cytometry on days 8, 10, 12 and 14 after exposure. Shown are mean value ± SD for three independent samples. GPI(−) cells frequency after 24 μM MMS exposure (b). Bars represent the GPI(−) frequency and lines represent relative increases in cells counts