Fig. 2

Exonucleolytic digestion in acetaldehyde-treated oligonucleotides. a Schematic drawing of ³² P -labelled 70-mer oligonucleotide. b Action of the exonuclease function of T4 DNA polymerase on an oligonucleotide containing acetaldehyde induced DNA lesions. Non-treated oligonucleotides (lanes 1–5) and acetaldehyde-treated oligonucleotides (lanes 6–10) were digested with increasing amounts of T4 DNA polymerase (0, 0.3, 0.75, 1.5, and 3 units) in the absence of deoxynucleoside triphosphates at 37 °C for 30 min