Fig. 3

DNA replication reaction in acetaldehyde-treated plasmids. a In the absence of DNA damage, phi29 DNA polymerase and random primers generate new DNA synthesis products from the template. If acetaldehyde damages DNA, the resulting lesions inhibit DNA synthesis, as phi29 DNA polymerase cannot synthesize new DNA products from damaged templates, and products will not be detected. b Agarose gel (1%) demonstrating the presence of an acetaldehyde-induced lesion. The phi29 DNA polymerase and non-acetaldehyde treated DNA template/random primer complexes (lane 1) or acetaldehyde treated DNA template/random primer complexes (lane 5) were incubated for the indicated times (0,1, 2, and 4 h: lanes 1–4 or lanes 5–8). Rp is random primers and triangles are incubation time. c Quantification of DNA synthesis products via 1% agarose gel analysis (b)