Fig. 4

RNA transcription reaction of acetaldehyde-treated plasmids. a In the absence of DNA damage, the T7 RNA polymerase generates RNA transcripts from DNA templates. After purifying RNA, real-time reverse transcription-PCR (qRT-PCR) is performed, and the PCR products are analyzed. If acetaldehyde damages DNA, the resulting lesions inhibit RNA synthesis, as T7 RNA polymerase cannot synthesize transcripts from damaged templates, and qRT-PCR products will not be detected. Amplification plot of qRT-PCR analysis of RNA transcripts of UV-irradiated (b) or of acetaldehyde (AA)-treated (c) DNA templates. UV-irradiated (d) or acetaldehyde-treated (e) pBSII was incubated with T7 RNA polymerase, and transcription was quantified by qRT-PCR