Bisphenol A enhances adipogenic signaling pathways in human mesenchymal stem cells

Genes and Environment

Table 1 The name and sequence of the primers, the sizes, and annealing temperatures for each pair

Gene	Size (bp)	Sequence (5′ → 3′)	Annealing temperature (°C)
GAPDH	113	F:CATGAGAAGTATGACAACAGCCT R:AGTCCTTCCACGATACCAAAGT	58
PPARγ	80	F:CAGAAATGCCTTGCAGTGGG R:AACAGCTTCTCCTTCTCGGC	59
CEBPα	94	F:TATAGGCTGGGCTTCCCCTT R:AGCTTTCTGGTGTGACTCGG	60
CEBPβ	154	F:TTTGTCCAAACCAACCGCAC R:GCATCAACTTCGAAACCGGC	59
SREBP1c	117	F:TCTCAGTCCCCTGGTCTCTG R:ATAGGCAGCTTCTCCGCATC	59
INSIG2	114	F:AGTGGTCCAGTGTAATGCGG R:TGGATAGTGCAGCCAGTGTG	60
LPL	137	F:GCTCAGGAGCATTACCCAGTGTC R:GCTCCAAGGCTGTATCCCAAGA	63
FASN	107	F:ATTCTGCCATAAGCCCTGTC R:CTGTGTACTCCTTCCCTTCTTG	57

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, PPARγ Peroxisome proliferator-activated receptor-gamma, C/EBPα CCAAT-enhancer-binding protein-alpha, C/EBPβ CCAAT-enhancer-binding protein- beta, SREBP1c Sterol regulatory element-binding protein-1c, INSIG2 Insulin induced gene-2, FASN Fatty acid synthase, LPL lipoprotein lipase

ISSN: 1880-7062