Fig. 2

Discrimination of FFPE-AAF from FFPE-CRE together with previous rat GTHCs, NGTHCs and NGTNHCs calculated from public Open TG-GATEs data [4] using PCA. FFPE data show individual results and TG-GATEs data show mean of three rats in each point. Red: FFPE-AAF, brown: AAF at 24 h from Open TG-GATEs, light brown: AAF on 29 days from Open TG-GATEs, black: GTHCs from Open TG-GATEs. Yellow: FFPE-CRE, blue: NGTHCs from Open TG-GATEs, light blue: NGTNHCs from Open TG-GATEs. Two points of FFPE-CRE (− 0.042/− 3.26 and − 0.08/− 3.26) overlapped in Fig. 2. PCA was conducted on data of the ratio (exp/cont) as in Additional file 4 (FFPE-AAF and FFPE-CRE together with previously calculated TG-GATEs data [4]). PC1 and PC2 of the results are shown in Additional file 5. Five typical GTHCs (AAF, AFL, DEN, 2NF and NNM at 24 h and AAF and DEN on 29 days in Open TG-GATEs data) were clearly separated from the seven typical NGTHCs (CLO, ETH, FEN, GEM, HEX, PHE and WY at 24 h and 29 days in TG-GATEs data) and eleven NGTNHCs (AA, ASP, CAF, CPA, CPP, DEX, DIA, IND, PBZ, THE and TOL at 24 h and 29 days in Open TG-GATEs data) using PCA. Two groups of GTHCs and (NGTHCs and NGTNHCs) were separated using PC1 (− 0.637 for DEN24L against − 0.159 for FEN24M. Dashed line is border line of the two groups. FFPE-AAF in GTHCs group was clearly separated from FFPE-CRE grouped in NGTHCs