Table 2 Mutagenic potencies of tested compounds in standard TA1535 and TA100 versus new Ames tester strains overexpressing the episomal Y-family DNA polymerases. Data from tests conducted in the presence of the lac promoter inducer as described under Material and Methods are added for comparison in the columns under the IPTG label. The lac promoter drives the expression of the activated forms of DNA polymerases RI* and SamA’B overexpressed in the strains YG9028 and YG9029, respectively
Compound | Specific mutagenicity [revertants/plate/nmole] | |||||||
|---|---|---|---|---|---|---|---|---|
TA1535 | TA100 | YG9028 | YG9029 | |||||
IPTG | IPTG | IPTG | IPTG | |||||
AF-2 *) | – | – | 18,788 | 16,381 | 10,697 | 5113 | 1961 | 3723 |
MMS | – | – | 0.103 | 0.099 | 0.205 | – | – | – |
AFB1 | – | – | 846 | 692 | – | – | – | – |
1,8-DNP | – | – | 38,465 | 53,033 | 2595 | neg. | 3782 | 14,195 |
AG | 1.047 | 1.151 | 0.817 | 0.646 | 0.42 | 0.128 | 0.753 | 0.464 |