Fig. 3

Expression of MAGE-A genes correlates with hypomethylation of promoter regions. a Number of fully methylated CpG sites 1000 bp upstream of transcription start site of each MAGE -A gene were counted using methylome datasets in the UCSC genome browser in the indicated tissue (H) heart, (T) testis and (S) Sperm. b Number of fully methylated CpG sites 1000 bp upstream of transcription start site of each MAGE -A gene were counted using methylome datasets in the UCSC genome browser in the indicated tissue, (N) normal colon, (AP) adenomatous polyp, (CC) colon cancer tissue. c Number of fully methylated CpG sites 1000 bp upstream of transcription start site of each MAGE -A gene were counted using two methylome datasets in the UCSC genome browser in HCT116 wild type cell line (WT) and HCT116 where DNMT1 and DNMT3b (DNA methyl transferases) have been depleted (DKO). d mRNA expression of the indicated MAGE gene in HEK cells treated with DMSO (black) or 100 nM Azacytidine (grey) or HCT116 cancer cell line (lined). Expression of each MAGE gene is normalized to expression in HEK cells. Data shown are mean ± S.D. from n = 3 experiments. P values were determined from Students' t-test and * p < 0.001 and # is not-significant (NS)