| S9 mix | Preincubation | Volume of acetone (μL) | Number of survivors (× 108 cells/mL) |
|---|
| TA100 | TA1535 | TA98 | TA1537 | WP2uvrA |
|---|
| without | Before | 0 | 4.13 ± 0.24 | 5.48 ± 0.22 | 4.90 ± 0.28 | 4.84 ± 0.24 | 3.02 ± 0.32 |
| | (100) | (100) | (100) | (100) | (100) |
| 25 | 4.08 ± 0.38 | 5.86 ± 0.15 | 4.69 ± 0.23 | 4.87 ± 0.20 | ND |
| | (99) | (107) | (96) | (101) | ND |
| 50 | 3.99 ± 0.23 | 5.52 ± 0.21 | 4.70 ± 0.19 | 4.80 ± 0.16 | 2.71 ± 0.20 |
| | (97) | (101) | (96) | (99) | (90) |
| After | 0 | 4.32 ± 0.16 | 6.02 ± 0.27 | 5.31 ± 0.21 | 4.56 ± 0.22 | 2.89 ± 0.10 |
| | (105) | (110) | (108) | (94) | (96) |
| 25 | 3.71 ± 0.24 | 5.50 ± 0.15 | 4.58 ± 0.13 | 4.03 ± 0.37 | ND |
| | (90) | (100) | (93) | (83) | ND |
| 50 | 2.70 ± 0.28 | 4.25 ± 0.26 | 4.29 ± 0.20 | 3.83 ± 0.18 | 2.86 ± 0.14 |
| | (65) | (78) | (88) | (79) | (95) |
| with | Before | 0 | 3.99 ± 0.22 | 5.29 ± 0.20 | 4.28 ± 0.26 | 3.51 ± 0.21 | 2.42 ± 0.53 |
| | (96) | (97) | (87) | (73) | (80) |
| 25 | 3.81 ± 0.23 | 4.87 ± 0.18 | 4.66 ± 0.16 | 3.21 ± 0.16 | 2.69 ± 0.23 |
| | (92) | (89) | (95) | (66) | (89) |
| 50 | 3.49 ± 0.16 | 4.70 ± 0.23 | 4.36 ± 0.33 | 3.04 ± 0.29 | 2.61 ± 0.25 |
| | (84) | (86) | (89) | (63) | (86) |
| After | 0 | 3.81 ± 0.25 | 5.01 ± 0.38 | 3.69 ± 0.28 | 2.23 ± 0.19 | 3.01 ± 0.66 |
| | (92) | (91) | (75) | (46) | (100) |
| 25 | 2.93 ± 0.13 | 3.92 ± 0.29 | 3.58 ± 0.14 | 1.56 ± 0.12 | 2.34 ± 0.60 |
| | (71) | (72) | (73) | (32) | (78) |
| 50 | 1.80 ± 0.07 | 2.78 ± 0.11 | 1.99 ± 0.11 | 1.16 ± 0.17 | 2.46 ± 0.18 |
| | (44) | (51) | (41) | (24) | (81) |
- ND; Not determined. The number of survivors was determined in a treatment mixture containing different amounts of acetone and 0.1 mL of bacterial culture with or without 0.5 mL of S9 mix, before or after preincubation at 37 °C for 20 min. The number of survivors indicated is the mean ± standard deviation of those obtained from duplicate test tubes and duplicate plates for each test tube. Figures in parentheses are ratio of the number of survivors expressd as a percentage of the number of survivors for those without acetone in the absence of S9 mix before preincubation
