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Table 2 Cytotoxicity of acetone on bacterial cells with or without S9 mix, before and after preincubation in the Ames preincubation test

From: Evaluation of acetone as a solvent for the Ames test

S9 mix

Preincubation

Volume of acetone (μL)

Number of survivors (× 108 cells/mL)

TA100

TA1535

TA98

TA1537

WP2uvrA

without

Before

0

4.13 ± 0.24

5.48 ± 0.22

4.90 ± 0.28

4.84 ± 0.24

3.02 ± 0.32

 

(100)

(100)

(100)

(100)

(100)

25

4.08 ± 0.38

5.86 ± 0.15

4.69 ± 0.23

4.87 ± 0.20

ND

 

(99)

(107)

(96)

(101)

ND

50

3.99 ± 0.23

5.52 ± 0.21

4.70 ± 0.19

4.80 ± 0.16

2.71 ± 0.20

 

(97)

(101)

(96)

(99)

(90)

After

0

4.32 ± 0.16

6.02 ± 0.27

5.31 ± 0.21

4.56 ± 0.22

2.89 ± 0.10

 

(105)

(110)

(108)

(94)

(96)

25

3.71 ± 0.24

5.50 ± 0.15

4.58 ± 0.13

4.03 ± 0.37

ND

 

(90)

(100)

(93)

(83)

ND

50

2.70 ± 0.28

4.25 ± 0.26

4.29 ± 0.20

3.83 ± 0.18

2.86 ± 0.14

 

(65)

(78)

(88)

(79)

(95)

with

Before

0

3.99 ± 0.22

5.29 ± 0.20

4.28 ± 0.26

3.51 ± 0.21

2.42 ± 0.53

 

(96)

(97)

(87)

(73)

(80)

25

3.81 ± 0.23

4.87 ± 0.18

4.66 ± 0.16

3.21 ± 0.16

2.69 ± 0.23

 

(92)

(89)

(95)

(66)

(89)

50

3.49 ± 0.16

4.70 ± 0.23

4.36 ± 0.33

3.04 ± 0.29

2.61 ± 0.25

 

(84)

(86)

(89)

(63)

(86)

After

0

3.81 ± 0.25

5.01 ± 0.38

3.69 ± 0.28

2.23 ± 0.19

3.01 ± 0.66

 

(92)

(91)

(75)

(46)

(100)

25

2.93 ± 0.13

3.92 ± 0.29

3.58 ± 0.14

1.56 ± 0.12

2.34 ± 0.60

 

(71)

(72)

(73)

(32)

(78)

50

1.80 ± 0.07

2.78 ± 0.11

1.99 ± 0.11

1.16 ± 0.17

2.46 ± 0.18

 

(44)

(51)

(41)

(24)

(81)

  1. ND; Not determined. The number of survivors was determined in a treatment mixture containing different amounts of acetone and 0.1 mL of bacterial culture with or without 0.5 mL of S9 mix, before or after preincubation at 37 °C for 20 min. The number of survivors indicated is the mean ± standard deviation of those obtained from duplicate test tubes and duplicate plates for each test tube. Figures in parentheses are ratio of the number of survivors expressd as a percentage of the number of survivors for those without acetone in the absence of S9 mix before preincubation