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Table 2 Cytotoxicity of acetone on bacterial cells with or without S9 mix, before and after preincubation in the Ames preincubation test

From: Evaluation of acetone as a solvent for the Ames test

S9 mixPreincubationVolume of acetone (μL)Number of survivors (× 108 cells/mL)
TA100TA1535TA98TA1537WP2uvrA
withoutBefore04.13 ± 0.245.48 ± 0.224.90 ± 0.284.84 ± 0.243.02 ± 0.32
 (100)(100)(100)(100)(100)
254.08 ± 0.385.86 ± 0.154.69 ± 0.234.87 ± 0.20ND
 (99)(107)(96)(101)ND
503.99 ± 0.235.52 ± 0.214.70 ± 0.194.80 ± 0.162.71 ± 0.20
 (97)(101)(96)(99)(90)
After04.32 ± 0.166.02 ± 0.275.31 ± 0.214.56 ± 0.222.89 ± 0.10
 (105)(110)(108)(94)(96)
253.71 ± 0.245.50 ± 0.154.58 ± 0.134.03 ± 0.37ND
 (90)(100)(93)(83)ND
502.70 ± 0.284.25 ± 0.264.29 ± 0.203.83 ± 0.182.86 ± 0.14
 (65)(78)(88)(79)(95)
withBefore03.99 ± 0.225.29 ± 0.204.28 ± 0.263.51 ± 0.212.42 ± 0.53
 (96)(97)(87)(73)(80)
253.81 ± 0.234.87 ± 0.184.66 ± 0.163.21 ± 0.162.69 ± 0.23
 (92)(89)(95)(66)(89)
503.49 ± 0.164.70 ± 0.234.36 ± 0.333.04 ± 0.292.61 ± 0.25
 (84)(86)(89)(63)(86)
After03.81 ± 0.255.01 ± 0.383.69 ± 0.282.23 ± 0.193.01 ± 0.66
 (92)(91)(75)(46)(100)
252.93 ± 0.133.92 ± 0.293.58 ± 0.141.56 ± 0.122.34 ± 0.60
 (71)(72)(73)(32)(78)
501.80 ± 0.072.78 ± 0.111.99 ± 0.111.16 ± 0.172.46 ± 0.18
 (44)(51)(41)(24)(81)
  1. ND; Not determined. The number of survivors was determined in a treatment mixture containing different amounts of acetone and 0.1 mL of bacterial culture with or without 0.5 mL of S9 mix, before or after preincubation at 37 °C for 20 min. The number of survivors indicated is the mean ± standard deviation of those obtained from duplicate test tubes and duplicate plates for each test tube. Figures in parentheses are ratio of the number of survivors expressd as a percentage of the number of survivors for those without acetone in the absence of S9 mix before preincubation