Fig. 2
A. Comparison of the relative expression of DNMT1, DNMT3A and DNMT3B in human endometrial stromal cells that were treated with 10− 11, 10− 8 and 10− 6 M of nicotine, measured by quantitative Real-time-PCR. Expression of each gene was normalized to β-Actin mRNA. The culture-media treated control cells were used as a reference, whose expression levels were set to 1.0, and expressions in all other cells were expressed as an n-fold difference relative to controls. Mean values ± SD of three experiments are given. Bars marked with different letters are significantly different as verified by Tukey’s honestly significant difference multiple comparison test (p < 0.05). B. A summary of the change in the average expression of all three DNMTs (T1/3A/3B) in HESC treated with 10− 11, 10− 8 and 10− 6 M of nicotine (p < 0.05)