Fig. 18

LNCaP cells (1 million cells) were incubated with test compounds HNOH-PhIP (10, 100 or 100 nM), DHT (1 nM), or t-BuOOH (500 μM) for 24 h [302]. Cells were lysed with 80% methanol/20% water containing 0.2% formic acid and extracts, concentrated by vacuum centrifugation, and analyzed by LC/MS with the Orbitrap Lumos at 120 K resolution [302]. Extracted ion chromatograms (5 ppm tolerance) for selected metabolite precursor ions and box and whisker plots depicting altered profiles for a glutamine, b glutamic acid, c GSH, d SAM, and e the product ion spectrum and the match to NIST 2019 mass spectrometry small molecule library entry supports the the identity of SAM