Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by the liquid chip technology

Chao, Guanqun; Wang, Qianqian; Ye, Fangxu; Zhang, Shuo

doi:10.1186/s41021-021-00205-2

Table 1 Target genes, amplicon sizes, labels, and sequences of primers and probes used in the Luminex assay

From: Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by the liquid chip technology

Target genes	Amplicon size (bp)	labels	Sequence (5′-3′)
HTR4	132	Forward	TCCTCATGGTGCTGGCCTAT
		Reverse	GATGTGTGCTGTGCTGGTCA
		Probe	AACGGGCAGGAGCCACCTCTGAAAGCA
HTR1a	122	Forward	CTCCACTTTCGGCGCTTTCT
		Reverse	CCGCTCCCTTCTTTTCCACC
		Probe	TCTTCAGAGCCGCGCGCTTCCGAAT
F2RL3	145	Forward	GTACTGTTCTCGGCAGTGGC
		Reverse	CTACGCAGCTGTTGAGGGTG
		Probe	TATGGAGCCTATGTGCCCAGCCTGGCA
NOS1	111	Forward	ATCAGCCAGCAAAGACCAGC
		Reverse	TTAGCCTGGGAGACTGAGCC
		Probe	TCCCAGTAACGGACCTCAGCATGCCCA
CALCA	103	Forward	AACACTGCCACCTGTGTGAC
		Reverse	AGGCTTCAGAGCCCACATTG
		Probe	ATCGGCTGGCAGGTCTGCTGAGCAGAT
NPY	132	Forward	CTGGAGGAGAGCTTGTGGGA
		Reverse	CGCCTACTCCACACTCCTCA
		Probe	ATCGGGCAGGAGCCACCGCTGAAATCA
CRHR2	94	Forward	TTGGATGGTGCATTCCCTGC
		Reverse	CCAGCTTCCTTGCCAAACCA
		Probe	TCATCGCCTGGGCAGTTGGCAAACTCT
IL1b	94	Forward	TCGCAGCAGCACATCAACAA
		Reverse	TCCACGGGAAAGACACAGGT
		Probe	TGGCTGTGGAGAAGCTGTGGCAGCT
P2RX3	93	Forward	GCTGGTATACGGGAACGCTG
		Reverse	AGAACAGTCCCCACTCCCAC
		Probe	ACCATCATCAGCTCGGTGGCTGCCTT
NOS2	80	Forward	ATCACTTCCCCGCCTAGTCC
		Reverse	TCTAGCTCCTGCTGTTCGCT
		Probe	TCGACTGCTCAGCACCCTGGCAGAAGA
TPH1	145	Forward	TGACATCTTCCCCCTGCTGA
		Reverse	CACTCTGTTGGCGCAGAAGT
		Probe	ACTCGCCCGATCAGCTCACTGCGAA
CRHR1	140	Forward	CCTGGCCAGCAATGTCTCTG
		Reverse	TGACGGCAATGTGGTAGTGC
		Probe	ATGGCTACCGGGAATGCCTGGCCAA
HMOX1	137	Forward	TCTGGTATGGGCCTCACTGG
		Reverse	GTCACCCAGGTAGCGGGTAT
		Probe	TCATCCCTTGCACGCCAGCCACACA
TRPV1	137	Forward	TCTGGTATGGGCCTCACTGG
		Reverse	GTCACCCAGGTAGCGGGTAT
		Probe	TCATCCCTTGCACGCCAGCCACACA
VIP	94	Forward	TGGAAGCCAGAAGCAAGCCT
		Reverse	GGTGGTCCAAAGAGAGGCCA
		Probe	AGTGTGCTGTTCTCTCAGTCGCTGGCC
F2RL1	102	Forward	TAATGGCATGGCCCTCTGGA
		Reverse	ATGACAGAGAGGAGGTCGGC
		Probe	ACACCCCGCCGTGATTTACATGGCCA
TGFB1	90	Forward	ATACGCCTGAGTGGCTGTCT
		Reverse	CGCTGAATCGAAAGCCCTGT
		Probe	ACGTCACTGGAGTTGTACGGCAGTGGC
HTR3a	141	Forward	ATAGACCCCCAGCCACCTTC
		Reverse	CCTCCCTTGGTGGTGGAAGA
		Probe	TCAGCCATGGGAAACCACTGCAGCCA
SLC6A4	91	Forward	CATCTTCACGGTGCTTGGCT
		Reverse	GATGAAAAGGAGGCTGGGGC
		Probe	ACGAAGACGTGTCCGAGGTGGCCAAA
TFF2	133	Forward	TCGGAGCAGTGTGTCATGGA
		Reverse	GTGGGAAGAAACACCAGGGC
		Probe	TCGCAAGAATTGTGGGTACCCGGGCA
AQP8	96	Forward	GGCCTTGGGGCTCATCATTG
		Reverse	TTGAGGCCTCCGATCACTGT
		Probe	TCAACCCTGCTGTGTCGCTGGCAGT

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ISSN: 1880-7062

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