Fig. 3

Suppression of elevated cellular oxidative stress in PRDX1-depleted cells by 2-mercaptoethanol. A Total cellular oxidative stress was detected by the oxidation of the fluorescent probe DCFH. B Cytoplasmic superoxide levels in PRDX1-depleted cells. Superoxide levels were measured with BES-So-AM. Cells were treated with or without 1 μg/ml Dox in the absence or presence of 50 mM 2-mercaptoethanol (Et-SH) for 96 h. SOD1-depleted cells were treated with 1 μg/ml Dox for 96 h used as a positive control in the cytoplasmic superoxide analysis (B). Data from flow cytometric analyses are shown as mean fluorescence intensity (MFI). MFI without the Dox treatment was used as the standard (arbitrary unit = 1). All data represent the mean ± standard deviation from three independent experiments. Asterisks (*) indicate p < 0.05 by a multiple-comparison one-way ANOVA (Tukey’s test)