Fig. 2

Micronucleus test in the glandular stomach, colon, bone marrow, peripheral blood, and liver with aneugens. The test was performed in rats administered COL (po), VBS (iv), and DOC (iv) for 4 days. The sampling points were as follows: 24 h after 4 daily administration (stomach, colon, bone marrow, and liver), and 24 h after the second administration (peripheral blood). Each bar represents the frequency of MNed cells or MNed immature erythrocytes (IMEs) (mean ± SD). Each closed circle represents %IME (mean ± SD). The horizontal axis represents chemical and/or dosage (mg/kg/day). N, Negative (vehicle) control; P, positive control (P1: MNU and DMH treatment, P2: MMC treatment); TOX, data were excluded due to severe toxicity; ND, not done. Statistical significance: **p < 0.01 (Kastenbaum & Bowman test), ^#p < 0.05 (Dunnett’s test), ^§p < 0.05 (Steel test), and ^‡‡p < 0.01 (Aspin-Welch test) compared to the negative control