Fig. 1

Hepatic PNPLA3 gene and SIRT1 protein expression and H3K9ac level of PNPLA3 promoter in C57BL/6 mice during the fasting/re-feeding transition. A Relative mRNA level of liver PNPLA3 measured by qPCR (N = 6 mice per group). B The recruitment of H3K9ac onto the PNPLA3 promoter detected by ChIP-qPCR using an anti-H3k9Ac antibody. Rabbit IgG was used as a mock antibody for negative control. The recruitment is expressed as fold enrichment over IgG (N = 4 mice per group). C The protein level of liver SIRT1 detected by western blot. Quantitative analysis of gray value on bands was performed using Image-Pro Plus software. Quantitative data are presented as the means ± SD (N = 3 independent experiments). Ctrl: mice fed a normal chow diet; Fasted: mice fasted for 24 h; Refed: mice refed a high sucrose diet for 12 h after 24 h of fasting. **P < 0.01, ***P < 0.001