Fig. 1

Effect of BPA and MBP on the GFP fluorescence levels driven by the FLO1 promoter. A DNMT yeast transformed with pF1GS was grown at the indicated concentrations of BPA in the absence of S-9 mix to OD600 of 1.6–2.7 or in the presence of S-9 mix to OD600 of 2.6–3.2. Then, the intensity of GFP was measured. B DNMT yeast transformed with pF1GS was grown at the indicated concentrations of MBP to OD600 of 1.0–2.6 in the absence of S-9 mix. Then, the intensity of GFP was measured. C DNMT yeast transformed with pF1GSTpA or p313eGFP was grown at the indicated concentrations of BPA in the presence of S-9 mix to OD600 of 2.0–3.0 (DNMT yeast harboring pF1GSTpA) or to OD600 of 2.0–3.1 (DNMT yeast harboring p313eGFP). Then, the intensity of GFP was measured. D DNMT yeast transformed with pF1GSTpA or p313eGFP was grown at the indicated concentrations of MBP in the absence of S-9 mix to OD600 of 1.1–2.7 (DNMT yeast harboring pF1GSTpA) or to OD600 of 0.9–1.9 (DNMT yeast harboring p313eGFP). Then, the intensity of GFP was measured. The data are presented as the means ± SEM from more than three independent experiments. Statistical analysis was performed using ANOVA, followed by Dunnett’s post hoc test. (*P < 0.05, **P < 0.01, ***P < 0.001 versus control)