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Table 1 The mutation frequency of HeLa cells treated with PALs

From: Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity

Treatment a), b)

H2O2 Concentration

(μM)

Mutation Frequency

(10−7)

Relative Mutation frequency

Lactec

N.D.c)

10.4 ± 0.7

1.00

16-fold diluted 5-min PAL

65.3

161.2 ± 9.4

15.50

32-fold diluted 10-min PAL

42.7

56.6 ± 1.9

5.44

5-min PAL with catalase

N.D.c)

9.2 ± 0.9

0.88

1.5-fold diluted 10-min PAL with catalase

N.D.c)

10.8 ± 1.3

1.04

  1. a)Each of the solution has been adjusted by adding 20% (v/v) of 0.1 M citrate buffer (pH 6.1) in advance
  2. b)Here the concentration of H2O2 in untreated 5-min and 10-min PAL were 1299, 1650 μM, respectively
  3. c)Not detected