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Fig. 7 | Genes and Environment

Fig. 7

From: Short-term in vivo testing to discriminate genotoxic carcinogens from non-genotoxic carcinogens and non-carcinogens using next-generation RNA sequencing, DNA microarray, and qPCR

Fig. 7

Discrimination of FFPE-AAF from FFPE-CRE [9] together with the previous rat GTHCs, NGTHCs, and NGTNHCs calculated from public Open TG-GATEs data [47] using PCA. FFPE data show individual results and TG-GATEs data show the mean of three rats at each point. Red: FFPE-AAF, brown: AAF at 24 h from Open TG-GATEs, light brown: AAF on 29 days from Open TG-GATEs, black: GTHCs from Open TG-GATEs. Yellow: FFPE-CRE, blue: NGTHCs from Open TG-GATEs, light blue: NGTNHCs from Open TG-GATEs. Two points of FFPE-CRE (− 0.042/ − 3.26 and − 0.08/ − 3.26) overlapped. Five typical GTHCs [2-acetamidofluorene (AAF), AFL, DEN, 2NF, and NNM at 24 h and AAF and DEN on 29 daysin Open TG-GATEs data] were separated from the seven typical NGTHCs (CLO, ETH, FEN, GEM, HEX, PHE, and WY at 24 h and 29 days in TG-GATEs data) and eleven NGTNHCs (AA, ASP, CAF, CPA, CPP, DEX, DIA, IND, PBZ, THE, and TOL at 24 h and 29 days in Open TG-GATEs data) using PCA. Two groups of GTHCs and (NGTHCs and NGTNHCs) were separated using PC1 (− 0.637 for DEN 24L against − 0.159 for FEN 24 M). The dashed line is the border line of the two groups. FFPE-AAF in the GTHCs group was separated from FFPE-CRE grouped in NGTHCs [9]

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Genes and Environment

ISSN: 1880-7062

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