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Fig. 2 | Genes and Environment

Fig. 2

From: The H2TH-like motif of the Escherichia coli multifunctional protein KsgA is required for DNA binding involved in DNA repair and the suppression of mutation frequencies

Fig. 2

Three types of recombinant KsgA and their purification. (a) Domain organization of full-length KsgA, KsgA (1-213), and KsgA (9-273). (b) Purification of E. coli full-length KsgA, KsgA (1-213), and KsgA (9-273). Escherichia coli BL21(DE3) carrying pET21a (+)-KsgA, pET21a (+)-KsgA (1-213), or pET21a (+)-KsgA (9-273) was induced by 0.1 mM IPTG at 20 °C for 16 h. Proteins were separated by 0.1% SDS-12% PAGE and stained with Coomassie Blue. Lane 1, molecular weight marker; lane 2, purified His-KsgA (19.5 μg); lane 3, purified His-KsgA (1-213) (7.3 μg); lane 4, His-KsgA (9-273) (85.3 μg)

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Genes and Environment

ISSN: 1880-7062

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