DNA polymerase κ suppresses inflammation and inflammation-induced mutagenesis and carcinogenic potential in the colon of mice

Table 3 gpt MF in the distal colon of untreated or DSS-treated Polk^+/+ and Polk^−/− female mice

Group	Genotype		Total population	Confirmed mutants^a	Mutant Frequency (× 10^–6)	Mutant Frequency (× 10^–6) (average ± S.D.)	Sequenced mutants	Corrected mutants^a	M F^a (× 10^–6)	M F (× 10^–6) (average ± S.D.)
3	Polk^+/+	0	990,000	4	4.04	5.2 ± 1.8	4	2	2.0	4.4 ± 2.0
		0	1,344,000	5	3.72		5	5	3.7
		0	786,000	4	5.09		4	4	5.1
		0	900,000	7	7.78		7	6	6.7
4	Polk^+/+	2	1,674,000	5	2.99	5.3 ± 3.0	5	5	3.0	4.0 ± 1.5
		2	816,000	2	2.45		2	2	2.5
		2	960,000	8	8.33		7	5	5.2
		2	966,000	7	7.25		7	5	5.2
7	Polk^−/−	0	2,940,000	27	9.18	6.8 ± 4.1	25	18	6.1	5.2 ± 3.0
		0	792,000	3	3.79		3	2	2.5
		0	1,014,000	3	2.96		3	3	3.0
		0	1,668,000	19	11.39		19	15	9.0
8	Polk^−/−	2	1,440,000	35	24.31	17.8 ± 12.3	34	15	10.4	10.3 ± 6.3
		2	1,566,000	16	10.22		15	15	9.6
		2	984,000	31	31.50		31	18	18.3
		2	3,762,000	19	5.05		19	11	2.9

^aGenomic DNA was extracted from distal portion of the large intestine of Polκ^+/+ and Polκ^−/− female mice and subjected to gpt assays. Initially isolated gpt mutants were confirmed by streaking them on agar plates containing 6-TG. Then, the "confirmed mutants" were subjected to DNA sequencing. When the mutations were identified, they were classified as "corrected mutants". "MF" was calculated by dividing the number of "corrected mutants" by the number of "total population"

ISSN: 1880-7062