Polymorphism in autophagy-related genes LRP1 and CAPZA1 may promote gastric mucosal atrophy

Genes and Environment

Table 1 Characteristics of tag SNP genotyping primers, anneaking temperature, and cycle numbers

Gene	SNP	Major > Minor		Sequence of primer pairs (5' to 3’)	Annealing temperature(℃)	Cycle numbers
LRP1	rs715948	T > C	Forward	TCCCTTTCCAGCCCTAAAAT	64.0	50
	rs715948	T > C	Reverse	GCCCCCAGTAAGTGGTGTAA	64.0	50
	rs1799986	C > T	Forward	CACCATAGCCAGCTTGTTCA	62.5	50
	rs1799986	C > T	Reverse	CGGAAGGTGGGCTGATAATA	62.5	50
	rs1800137	C > T	Forward	AAGCTCGTCGACAGCAAGAT	63.5	50
	rs1800137	C > T	Reverse	GTTCCCCTACAGAGGCTTCC	63.5	50
	rs1800159	G > A	Forward	GCTAGCCAGGTGAGGCTGT	66.0	50
	rs1800159	G > A	Reverse	GGTGTGGAGGTGTCTGTGTG	66.0	50
	rs11172113	T > C	Forward	ACTCAGAATGGAAGCGGAGA	61.0	50
	rs11172113	T > C	Reverse	AGAGCCTGCAGGAATCTGAA	61.0	50
	rs11837145	C > A	Forward	CACAGGTCCCATCCAAGTG	62.5	50
	rs11837145	C > A	Reverse	CTCCAGGCCATTCAAAGGTA	62.5	50
CAPZA1	rs4423118	G > A	Forward	GATCAGAAAGGCAGCGACTC	62.0	50
	rs4423118	G > A	Reverse	CTGCAAGTTTTACCCCAAGG	62.0	50
	rs4525162	C > T	Forward	CTCCCCTGGGAGGTCATTAT	62.5	50
	rs4525162	C > T	Reverse	ACCTCTGTTGGGTGAAGTGG	62.5	50
	rs10885188	G > A	Forward	GTGTTGCTTCTATGTGCCTTTG	62.0	50
	rs10885188	G > A	Reverse	AACAGCCTTCTGCAGCAAAT	62.0	50
	rs58618380	T > A	Forward	AAGCTCTGTTTGGAGGCAAG	61.0	50
	rs58618380	T > A	Reverse	GAGCTTCCTTTGCCAGTTTG	61.0	50
	rs71481135	T > C	Forward	GGAGTGCAATGTCTGCTGAG	62.5	50
	rs71481135	T > C	Reverse	CCTCACCCATGATAGGCAGT	62.5	50
LAMP1	rs9577230	T > C	Forward	TGCAAGTTCTAGCCGGTTTT	61.0	50
	rs9577230	T > C	Reverse	AGGGTCTGCAACACACACAG	61.0	50
	rs9577502	A > T	Forward	GACATGCAATGCAAACACTG	62.0	50
	rs9577502	A > T	Reverse	TTTGGAGATGGTCTCGCTCT	62.0	50
	rs12871648	A > C	Forward	AGCCAGGCTTTTGGGTTTAT	65.6	50
	rs12871648	A > C	Reverse	CAAATTGGGTGGGAGATGAG	65.6	50

ISSN: 1880-7062