Fig. 1

Electropherograms for novel frameshift mutations in Romanian patients with CRC. A: c.165delT mutation, the top graph corresponds to case RC21 with mixed electropherogram (see the right side of highlighted area marked by an arrow) secondary to deletion of T. The middle electropherogram shows the same case after cloning of the corresponding PCR product and this cloning allowed to confirm the deletion of T (at the gap space highlighted by a vertical dark column). The lower graph corresponds to a wild type case (a control case without mutation). B: c.928_935dup mutation, the top electropherogram corresponds to case RC33 with a sequence of 8 bases that is duplicated; marked by a horizontal black line with a star on top and it was obtained after cloning of its PCR product in plasmid. The lower electropherogram corresponds to a wild type case (a control case without mutation)