Table 1 Primer pairs used for screening of EGFR exon19 rs121913438, exon21 rs121434568 mutation by ARMS-PCR
Mutation | Primer sequence | Size (bp) |
|---|---|---|
EGFR rs121913438 (Exon 19) | Common F (FC): 5`- GTAAATCCACCCAGATCACTG -3` Common R (RC): 5`- GTGTCAAGAAACTAGTGCTGGG -3` | 444 bp |
FM: 5`- GTTGGCTTTCGGAGATGTTTTGATAG -3` RN: 5`- CCCGTCGCTATCAAGGAATTAA -3` | 134 bp | |
325 bp | ||
EGFR rs121434568 (Exon 21) | FC: 5`—TGACCCTGAATTCGGATGCA -3` RN: 5`- TTCCGCACCCAGCAGTTTGGCTA -3` | 199 bp |
FC: 5`—TGACCCTGAATTCGGATGCA -3` RM: 5`- CGCACCCAGCAGTTTGGTT -3` | 196 bp |