Table 2 Optimization of PCR condition for EGFR (exon19) and (exon 21)
VARIANT NAME | Cycle name | Temperature °C | Time | Number of Cycles |
|---|---|---|---|---|
EGFR (Exon 19) rs121913438 | Initial denaturation | 95 | 5 min | 1 |
Denaturation | 95 | 35 s | 35 | |
Annealing | 65 | 35 s | ||
Extension | 72 | 40 s | ||
Final extension | 72 | 5 min | 1 | |
Soak | 4 | ∞ | 1 | |
EGFR (Exon 21) rs121434568 | Initial denaturation | 95 | 5 min | 1 |
Denaturation | 94.5 | 40 s | 35 | |
Annealing | 61.5 | 40 s | ||
Extension | 72 | 50 s | ||
Final extension | 72 | 5 min | 1 | |
Soak | 4 | ∞ | 1 |