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Table 2 List of strains used in the Ames test

From: Carcinogenic risk of food additive AF-2 banned in Japan: a case study on reassessment of genotoxicity

Strains

Characteristics

Genetic variation

Source

S. typhimurium

NR

pKM101

 

TA1535

hisG46 a), gal, Δ(chl, uvrB, bio) rfa

Wild type

No

[26]

TA100

Same as TA1535, but harbours pKM101; Apr b)

Wild type

Yes

[10]

TA1538

Same as TA1535, but hisD3052 c) instead of hisG46

Wild type

No

[26]

TA98

Same as TA1538, but harbours pKM101; Apr

Wild type

Yes

[10]

YG7127

Same as TA1535, but an nfsB gene is deleted; Kmr d)

Deleted

No

[20]

YG7128

Same as YG7127, but harbours pKM101; Apr, Kmr

Deleted

Yes

[20]

YG7131

Same as TA1538, but an nfsB gene is deleted; Kmr

Deleted

No

[20]

YG7132

Same as YG7131, but harbours pKM101; Apr, Kmr

Deleted

Yes

[20]

  1. a) hisG46 is a base-substitution mutation, CTC to CCC, at the 69th codon of the hisG gene
  2. b) Apr means ampicillin resistant
  3. c) hisD3052 is a frameshift mutation, CGCGCG to CGCGCGCG, in the hisD gene
  4. d) Kmr means kanamycin resistant