Table 1 Set of MRM transitions
From: Absolute quantification of DNA damage response proteins
Protein | Internal standard | Amino acid sequence of target peptide for quantification | MRM transition (m/z Q1 > Q3) | Collision energy (eV) |
|---|---|---|---|---|
H2AX | ATQASQEY | 449.2 > 526.2 | 10 | |
γH2AX | ATQApSQEY | 489.2 > 440.1 | 10 | |
MDC1 | VLFTGVVDAR | 539.0 > 717.2 | 25 | |
53BP1 | VITDVYYVDGTEVER | 344.6 > 459.5 | 11 | |
NBS1 | TTTPGPSLSQGVSVDEK | 852.1 > 577.6 | 30 | |
MRE11 | GNDTFVTLDEILR | 747.1 > 530.3 | 34 | |
RAD50 | GQDIEYIEIR | 618.3 > 693.1 | 21 | |
RAD51 | YALLIVDSATALYR | 785.3 > 461.5 | 24 | |
ATR | APLNETGEVVNEK | 700.3 > 875.5 | 23 | |
ATM | YLNWDAVFR | 592.3 > 492.6 | 22 | |
RPA1 | VIDQQNGLYR | 603.5 > 622.5 | 22 | |
XRCC5 | EEASGSSVTAEEAK | 698.0 > 834.5 | 22 | |
XRCC6 | SDSFENPVLQQHFR | 852.4 > 512.9 | 32 | |
H2AX | ○ | [13C3,15N]ATQASQEY | 451.2 > 526.2 | 10 |
γH2AX | ○ | [13C3,15N]ATQApSQEY | 491.2 > 442.1 | 10 |
MDC1 | ○ | VLFTGVVDA[13C6,15N4]R | 544.0 > 727.2 | 25 |
53BP1 | ○ | VITDVYYVDGTEVE[13C6,15N4]R | 349.5 > 469.5 | 11 |
NBS1 | ○ | TTTPGPSLSQGVSVDE[13C6,15N2]K | 856.1 > 585.6 | 30 |
MRE11 | ○ | GNDTFVTLDEIL[13C6,15N4]R | 752.1 > 540.3 | 34 |
RAD50 | ○ | GQDIEYIEI[13C6,15N4]R | 623.3 > 703.1 | 21 |
RAD51 | ○ | YALLIVDSATALY[13C6,15N4]R | 790.3 > 471.5 | 24 |
ATR | ○ | APLNETGEVVNE[13C6,15N2]K | 704.3 > 883.5 | 23 |
ATM | ○ | YLNWDAVF[13C6,15N4]R | 597.3 > 502.6 | 22 |
RPA1 | ○ | VIDQQNGLY[13C6,15N4]R | 608.5 > 632.5 | 22 |
XRCC5 | ○ | EEASGSSVTAEEA[13C6,15N2]K | 702.0 > 842.5 | 22 |
XRCC6 | ○ | SDSFENPVLQQHF[13C6,15N4]R | 857.4 > 522.9 | 32 |