Fig. 2

Results of genotoxicity tests for the selective CDK4/6 inhibitor PD0332991. a Ames test. Each dot indicates the mean of 2 plates and * indicates growth inhibition. b In vitro micronucleus test. Black dots indicate cytotoxicity, expressed as CBPI relative to the negative control (100%). Gray bars indicate the micronucleus frequency. * indicates a statistically significant difference (*: Fisher’s exact test and Bonferroni’s correction. Significance level was 2.5% one-sided). c Comet assay. Gray bars indicate the mean %DNA in tail and error bars indicate standard deviation. * indicates a statistically significant difference (*: Dunnett’s test compared with the vehicle control. Significance level was 5%). d In vivo chromosome aberration test. White circles indicate the individual number of bone marrow nucleated cells. Black bars indicate the mean structural aberration frequency and error bars indicate standard deviation. Gray bars indicate the mean polyploidy frequency and error bars indicate standard deviation. † and * indicate statistically significant differences (†: Dunnett’s test compared with the vehicle control. Significance level was 5%, *: Fisher’s exact test and Bonferroni’s correction compared with the vehicle control. Significance level was 2.5% one-sided)