Application of image-recognition techniques to automated micronucleus detection in the in vitro micronucleus assay

Table 1 Comparison of the count results of the manual counting and the MN/cell detection software

Sample	(µg/mL)	Number of photographs	Manual counting			Cell/MN counting software									Cell viability
			Manual counting			Initial analysis			Individual reanalysis			Batch reanalysis
			Total cells	MN cells	MN	Total cells	MN cells	MN	Total cells	MN cells	MN	Total cells	MN cells	MN
			(n)	(n)	(%)	(n)	(n)	(%)	(n)	(n)	(%)	(n)	(n)	(%)	(%)
Control	0	45	1555	11	0.71	1548	13	0.84	1549	16	1.03	1593	23	1.44	100
H₂O₂	3.75	55	1340	35	2.61	1352	155***	11.46	1352	32	2.37	1352	25	1.85	71
	7.5	57	1292	68	5.26	1336	91*	6.81	1297	72	5.55	1334	60	4.50	66
	15	43	1250	95	7.60	1262	93	7.37	1262	93	7.37	1262	93	7.37	84
	30	79	2040	189	9.26	2053	257*	12.52	2055	197	9.59	2093	197	9.41	75
K₂CrO₄	1.2	50	1353	36	2.66	1354	30	2.22	1354	31	2.29	1348	32	2.37	78
	2.4	60	1436	91	6.34	1408	87	6.18	1408	89	6.32	1413	84	5.94	69
	4.9	66	1270	139	10.94	1280	98*	7.66	1274	129	10.13	1299	118	9.08	56
MMC	0.0125	49	1919	62	3.23	1901	76	4.00	1896	68	3.59	1910	78	4.08	113
	0.025	36	1661	69	4.15	1679	112**	6.67	1676	72	4.30	1688	62	3.67	134
	0.05	30	1465	199	13.58	1456	225	15.45	1456	198	13.60	1497	183	12.22	141
	0.1	80	1203	472	39.24	1172	464	39.59	1172	455	38.82	1182	448	37.90	44
	0.2	65	1684	326	19.36	1682	378	22.47	1688	319	18.90	1704	321	18.84	75
MMS	10	49	1658	76	4.58	1642	80	4.87	1639	68	4.15	1647	90	5.46	98
	20	50	1147	106	9.24	1145	115	10.04	1143	116	10.15	1123	113	10.06	66
	40	73	1347	250	18.56	1363	257	18.86	1359	239	17.59	1361	257	18.88	53
	80	187	1155	50	4.33	1136	135***	11.88	1136	52	4.58	1149	58	5.05	18

MN (%) meant the percentage of micronucleus-containing cells in the total cells
^*: p < 0.05, **: p < 0.01, ***: p < 0.005 showed significant differences between the number of detections by the MN/cell detection software and the number of detections by manual counting in the same chemical concentration image group by Student’s t-test

ISSN: 1880-7062