Mutations induced by 8-hydroxyguanine (8-oxo-7,8-dihydroguanine), a representative oxidized base, in mammalian cells
© The Author(s) 2016
Received: 26 April 2016
Accepted: 15 June 2016
Published: 1 December 2016
Guanine oxidation occurs in both DNA and the cellular nucleotide pool, and one of the major products is 8-hydroxyguanine (8-oxo-7,8-dihydroguanine). The mutagenic potentials of this oxidized base have been examined in various experimental systems. In this review, we summarize the mutagenicity of the base in mammalian cells. We also describe the effects of specialized DNA polymerases, DNA repair proteins, and nucleotide pool sanitization enzymes.
Keywords8-Hydroxyguanine 8-Oxo-7,8-dihydroguanine Specialized DNA polymerase DNA repair protein Nucleotide pool sanitization enzyme
DNA oxidation by reactive oxygen species has been studied for decades, due to the pivotal role of damaged DNA in processes such as mutagenesis, carcinogenesis, aging, and neurodegeneration [1–3]. Moreover, oxidized DNA precursors (2’-deoxyribonucleoside 5’-triphosphates) formed in the cellular nucleotide pool also participate in these events. Reactive oxygen species are formed endogenously and are also produced by many environmental mutagens and carcinogens. Cancer involves multiple mutations in oncogenes and tumor suppressor genes, and thus oxidized DNA and DNA precursors might contribute to an extremely high percentage of carcinogenic events.
Kasai et al. reported the formation of 8-hydroxyguanine (GO, 8-oxo-7,8-dihydroguanine) by the oxidation of guanine in DNA and nucleosides [4–6]. Moreover, many research groups observed its generation under various experimental conditions in vitro, in living cells, and in vivo (e.g. [7–11]). GO is now recognized as one of the most important DNA lesions and has been used as a marker for DNA oxidation [12, 13], due to its prevalence in DNA and its high mutagenicity in mammalian cells. In this review, we summarize the mutagenic properties of the GO damage in mammalian cells, based on results obtained in various experimental systems.
Targeted mutations induced by GO
One of us (HK) constructed synthetic c-Ha-ras proto-oncogenes containing an GO:C pair in hotspots (codons 12 and 61). The modified bases were introduced into the first and second positions of codon 12 in the sense strand (5’-GGC-3’), and into the first position of codon 61 in the antisense strand (5’-CAA-3’, antisense strand 5’-TTG-3’) of the gene. Since most amino acid alterations in these hotspots activate the gene [14, 15], most base substitution mutations at the GO sites induce the transformation of mouse NIH3T3 cells. Thus, the type of mutation induced by the damaged base was determined by an analysis of the gene present in focus-forming cells, after transfection into NIH3T3 cells [16, 17]. The numbers of foci formed upon the transfection of the c-Ha-ras genes with GO were ~1 %, as compared to those formed by the activated c-Ha-ras genes (Val/Asp-12 and Lys/His-61).
The mutagenicity of GO in double-stranded (ds) plasmid DNA has been examined. Le Page et al. constructed a shuttle plasmid containing an GO:C pair in the sequence corresponding to codon 12 of the human c-Ha-ras gene (5’-GGOC-3’), and introduced it into simian COS-7 and human MRC5V1 cells . The vector has the SV40 origin and can replicate in these cell lines. The replicated plasmid DNA was recovered and introduced into bacterial cells. The plasmid DNAs isolated from colonies were analyzed by a restriction enzyme that cleaves the plasmid without the targeted mutations and by sequencing. Among the 101 bacterial colonies analyzed, none had the mutated sequence in the COS-7 experiment. Moreover, only one colony among the 125 colonies obtained in the MRC5V1 experiment had the targeted G➔T transversion. Thus, the mutation frequency of GO was less than 1 % in their experiments.
Our research group also performed similar experiments, using a shuttle vector containing the oxidized base in the supF gene and the SV40 origin [26–30]. The base was incorporated into the 5’-GGOT-3’ sequence of the gene. The GO plasmid was transfected into human 293T and U2OS cells, and the replicated plasmid was introduced into the indicator bacterial cells (KS40/pOF105). Mutations in the supF gene are detectable using this strain, since most mutations in the gene confer nalidixic acid and streptomycin resistance to the cells. The frequencies of base substitution mutations at the GO site were 3–6 and ~1 × 10-3 in 293T and U2OS cells, respectively. The major mutation was a G➔T transversion, but G➔A and G➔C mutations were also observed at lower frequencies. Semitargeted mutations at the 5’-flanking positions were also detected.
Sunaga et al. and Yamane et al. incorporated an GO:C pair into the 5’-AGOG-3’ sequence in the supF gene, and introduced the ds supF shuttle plasmid into NCI-H1299 cells [31, 32]. In contrast to the experiments by Le Page et al. and our research group, the G➔T mutations were induced with frequencies of 2-4 %.
Yasui et al. developed a system for the site-specific introduction of DNA lesions into human genomic DNA . They used lymphoblastoid TSCER122 cells heterozygous for the thymidine kinase gene. The cells display a TK-/- phenotype, since one allele contains a mutation in exon 4 and the other has an I-Sce I site and a 356-bp deletion in exon 5. Linear 6.1-kbp DNA containing the wild-type exon 5 and an GO residue (in an intron) was introduced into the cells, together with the I-Sce I expression DNA. The correct targeting restored the wild-type phenotype for thymidine kinase. Sequence analysis of the genomic DNA of revertant clones (TK+/-) indicated that the modified base induced G➔T, G➔C, and G➔A targeted mutations, with G➔T mutation frequencies of 5-8 %.
In addition to the approaches using ds DNA, the mutagenicity of GO in single-stranded shuttle plasmid (phagemid) DNAs has been examined in simian cells [34–36]. GO induced mutations with a frequency of 4–7 %, and the major mutation was the G➔T transversion. Moreover, G➔A and G➔C mutations were detected. Recently, Pande et al. examined the mutagenic properties of GO in the 5’-TGON-3’ (N = A, G, C, and T) sequence in human 293T cells . The frequencies of G➔T targeted mutations were 5–11 %, and G➔A and 2-base deletion mutations were also induced. In particular, the G➔A mutation was observed as frequently as the G➔T mutation, in the case of the 5’-TGOG-3’ sequence. In addition to the mutations in the modified position, semi-targeted mutations at the neighboring positions were found.
Roles of specialized DNA pols
Expected functions of cellular proteins related to mutations by GO directly produced in DNA
Specialized DNA pol
error-free bypass, suppression of untargeted mutations
suppression of G→T mutation by GO removal
suppression of G→T mutation by A removal
suppression of G→T mutation
suppression of G→T mutation
suppression of untargeted mutations
Interestingly, the knockdown of DNA pol λ increased the 2-base deletion mutations induced by GO in single-stranded DNA . This result suggested that DNA pol λ is involved in the error-free bypass of GO, by preventing the 2-base deletion.
Roles of DNA glycosylases
Overexpression of nuclear OGG1 or MUTYH, which are the major DNA glycosylases involved in the base excision repair of GO , suppressed the G➔T mutation frequency in a ds supF shuttle plasmid containing an GO:C pair [31, 32]. Yasui et al. also reported that the G➔T mutation, caused by GO site-specifically introduced into genomic DNA, was reduced in MUTYH-overexpressing cells . In accordance with these observations, we found that the knockdowns of the OGG1 and MUTYH DNA glycosylases in human cells significantly increased the frequencies of G➔T transversion caused by GO:C in the supF shuttle plasmid . Surprisingly, the G➔T mutation was also enhanced when the levels of other DNA glycosylases, NTH1 and NEIL1, were decreased. These results indicated that all of these DNA glycosylases suppress the G➔T mutations caused by GO:C pairs generated in DNA (Table 1).
Untargeted mutations induced by GO
Our research group introduced the ds supF plasmid containing GO:C into human U2OS cells, in which the Werner syndrome protein (WRN) was knocked-down. The total supF mutant frequency was 1.6-fold higher in the knockdown cells, as compared to the control cells . Sequence analysis indicated that the targeted G➔T mutation frequency was increased only slightly by the WRN knockdown. Instead, the knockdown promoted base substitution mutations at untargeted G (or G:C) sites with statistical significance. These “action-at-a-distance mutations” seemed to be broadly distributed throughout the supF gene. As discussed in the original report, there are many possible explanations for these types of mutations at this time.
Similar “action-at-a-distance mutations” were observed when the GO:C plasmid was transfected into cells with knocked-down DNA pol λ, one of the specialized DNA pols . The untargeted mutations at the G sites were significantly increased, but the frequency of untargeted mutations at G:C pairs was not significant (P = 0.10). The cause(s) of the untargeted mutations remain unknown, but they could be the same as those observed in the WRN knockdown cells, since DNA pol λ interacts with WRN .
As mentioned above, substitution mutations were found at the 5’-adjacent positions of GO [16, 17]. Interestingly, Nishimura and his colleagues found that human DNA pol η misincorporated deoxyribonucleotides opposite G at the 5’-flanking site of GO in the 5’-GGOC-3’ sequence in vitro . The 5’-flanking mutations observed in mammalian cells may involve some similar events.
Mutations induced by 8-OH-dGTP in mammalian cells
Roles of specialized DNA pols in mutagenesis by dGOTP
Expected functions of cellular proteins related to dGOTP-induced mutation
Specialized DNA pol
incorporation of dGOTP
incorporation of dGOTP
incorporation of dGOTP
promotion of A:T→C:G mutation
Nucleotide pool sanitization enzyme
decrease of dGOTP
decrease of dGOTP
decrease of dGOTP
Meanwhile, no obvious effects were observed when plasmid DNA containing GO:A was transfected into DNA pol ζ- and REV1-knockdown cells. Thus, the two DNA pols are likely to play important roles in the incorporation of dGOTP, but not in the insertion of dCTP opposite GO (Table 2).
Mutation enhancement by MUTYH DNA glycosylase
The MUTYH DNA glycosylase removes A paired with GO, thus preventing G➔T mutations . However, this activity may promote the A:T➔C:G transversions induced by dGOTP. We examined this possibility by the knockdown of the enzyme and the subsequent introduction of dGOTP or a supF plasmid containing an GO:A pair into the cells . The A:T➔C:G mutation frequency of the shuttle plasmid containing A paired with GO was decreased in the MUTYH-knockdown cells. The knockdown of MUTYH also reduced the mutation frequency induced by the introduction of dGOTP into cells . These results suggested that MUTYH promotes A:T➔C:G mutations by dGOTP in the nucleotide pool, although it suppresses G➔T mutations induced by GO formed by the direct oxidation of DNA (Fig. 2 and Table 2).
No effects were observed when dGOTP or a supF plasmid containing GO:A was introduced into the cells in which the OGG1, NTH1, and NEIL1 glycosylases were knocked-down .
Nucleotide pool sanitization enzymes
Nucleotide pool sanitization, the specific hydrolysis of damaged DNA precursors, is an important means by which organisms prevent mutations [48, 49]. In mammalian cells, the MTH1 (NUDT1), MTH2 (NUDT15), and NUDT5 proteins catalyze the hydrolysis of dGOTP and/or its diphosphate derivative to produce the monophosphate compound [50–53]. Both the supF plasmid DNA and dGOTP were introduced into cells in which the expression of each protein was knocked-down. The A:T➔C:G substitution mutations induced by dGOTP were higher in the knockdown cells than in control cells . The increase in the induced mutation was more evident in the triple knockdown cells. These results indicated that all three proteins act as a defense against the mutagenesis induced by oxidized dGTP (Table 2).
The G➔T transversion is the major targeted substitution mutation caused by the GO base in mammalian cells. Moreover, the G➔A and G➔C mutations at the GO site and the substitution mutations at the 5’-adjacent position of GO are also induced. Action-at-a-distance mutations at untargeted positions are detected when the levels of WRN and DNA pol λ are reduced. The A:T➔C:G (A➔C) transversion is the mutation induced by dGOTP in the cellular nucleotide pool. The DNA repair and nucleotide pool sanitization enzymes function as the defenses against GO in DNA and the nucleotide pool, respectively. MUTYH is an exceptional DNA repair protein, since it enhances the A:T➔C:G mutations when GO is formed in the nucleotide pool. Some specialized DNA pols are involved in nucleotide incorporation opposite GO and/or dGOTP incorporation, and thus affect the mutation induction by GO. The mutagenic properties of GO are affected by various factors, including the sequence contexts and the amounts of specialized DNA pols, DNA repair proteins, and nucleotide pool sanitization enzymes. This is one of the explanations for the fact that various mutation frequencies of GO:C have been observed, as described above. Further studies are necessary to reveal the detailed mechanisms of the GO-induced mutagenesis and its suppression by cellular proteins, using various experimental systems.
dGOTP, 8-hydroxy-dGTP (8-oxo-7,8-dihydro-dGTP); GO, 8-hydroxyguanine (8-oxo-7,8-dihydroguanine); pol, polymerase; ds, double-stranded.
The authors wish to thank the collaborators who participated in the experiments described in this paper, especially Profs. Eiko Ohtsuka and Hideyoshi Harashima of Hokkaido University. Work in our laboratory was supported in part by the Japan Society for the Promotion of Science KAKENHI Grants 20012001, 25550032 and 16H02956, and Grants from the Takeda Science Foundation to HK.
Both TS and HK wrote the manuscript and read and approved the final version.
The authors declare that they have no competing interests.
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