Fig. 3
Mutant frequencies of gpt induced by AAI in the kidney and liver of mice. Eight-week-old, male, WT and HRN gpt delta mice were administrated AAI once a week for 4 weeks. One week after the last dose, the mice were euthanized. The liver and kidney were taken and quickly frozen in liquid nitrogen, then kept in freezer at −70 °C until being analyzed. a, the mutant frequencies of gpt gene in the kidney of mice. The mutant frequencies of gpt in kidney for WT Control, HRN gpt delta Control, WT-AAI and HRN-AAI were 2.49 ± 0.74 × 10−6, 4.52-1.37 × 10−6, 121.27 ± 38.48 × 10−6 and 271.96 ± 47.59 × 10−6 respectively. b, the mutant frequencies of gpt in the liver of mice. The mutant frequencies of gpt in liver for WT Control, HRN Control, WT-AAI and HRN-AAI were 2.03 ± 1.78 × 10−6, 5.71 ± 2.71 × 10−6, 9.26 ± 9.73 × 10−6 and 32.81 ± 32.05 × 10−6 respectively. Values presented are the mean ± SD, N = 5