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Association between ACTN3 and acute mountain sickness

Genes and Environment volume 41, Article number: 18 (2019) Cite this article

Abstract

Background

During the process of acclimatization, when our organism needs to adjust several metabolic processes in the attempt of establishing a better oxygenation, it is normal that individuals present some symptoms that can lead to the disease of the mountain. However, not everyone presents such symptoms and individuals native of high altitudes regions present genetic differences compared to natives of low altitudes which can generate a better acute adaptation. One of these differences is the higher proportion of type I muscle fibers, which may originate from the R577X polymorphism of the ACTN3 gene. The aim of this study was to compare the response of individuals with different ACTN3 genotypes at simulated 4500 m altitude on the presence of Acute Mountain Sickness (AMS) symptoms. Twenty-three volunteers (RR = 7, RX = 8, XX = 8) spent 4 hours exposed to a simulated altitude of 4500 m inside a normobaric hypoxia chamber. Lactate and glucose concentrations, SpO2, heart rate and the symptoms of AMS were analyzed immediately before entering the chamber and at each hour of exposure. Statistical analysis was performed using IBM SPSS Statistics 21 software.

Results

Our results point to an association between AMS symptoms and the presence of R allele from R577X polymorphism.

Conclusion

We conclude that individuals with at least one R allele of the R577X polymorphism seems to be more susceptible to the effects of hypoxia during the acclimatization process and may develop AMS symptoms.

Introduction

While we ascend high altitudes, the pressure of oxygen present in the air descends. This phenomenon is known as hypobaric hypoxia and results in decreased pressure of oxygen in arterial blood, which is called hypoxemia [1]. An environment of hypobaric hypoxia, as found at high altitudes, affects several body systems even if exposure to this environment is acute (7 days) [2, 3]. The simple fact of ascending and exposing itself to a certain altitude causes our body to promptly initiate a series of adaptations to compensate for the decrease in the supply of oxygen [1, 4, 5], such as increased heart rate [6], use of glucose as substrate [7, 8] and consequent increase in lactate [9]. At 4500 m, the real amount of oxygen in the air composition is only 12% diluted, which is approximately 60% of sea level oxygen [10]. This lower oxygen pressure causes arterial desaturation (hypoxemia) that is perceived by peripheral chemoreceptors [11] causing increased ventilation and increased sympathetic activation [12], raising heart rate and cardiac output [13]. There are many people who seek high altitudes, whether for work, as miners in Chile and Peru that reach 4500 m [14] or only for sport or entertainment [15]. This high frequency of people at high altitudes between 4500 m and 5000 m brings concern, since 50% of them end up suffering from AMS symptoms [16], which involves headache, fatigue, dizziness, anorexia and nausea. Headache is the most frequent symptom [17], caused mainly by the decrease in arterial oxygen saturation [18,19,20]. The AMS diagnosis is made by a self-assessment questionnaire called the Lake Louise Score [21]. For the diagnosis it is necessary that the individual is above 2500 m and has the presence of at least two symptoms, one of them being headache [17]. The factors that predispose individuals to AMS symptoms are focus of several studies, including the search for some genetic relation, but without any conclusive results [22]. There are still many questions to be answered about the effects of different altitudes in the acclimatization process, but the literature helps to understand the chronic effects of hypoxemia, especially when analyzing populations living in high altitude regions. The Sherpas, Tibetans [23] and Quechuas [24] have a higher proportion of type I muscle fibers than individuals born in lower regions. These fibers have a higher mitochondrial density than type II fibers and this relationship between the distribution of muscle fiber types and populations born at high altitudes presupposes a genetic predisposition. In addition to the mitochondrial density difference between muscle fiber types, there is a structural difference in the sarcomere of these fibers. The actin filament binds to the Z-line by a protein that is found in two forms in skeletal muscle: α-actinin (ACTN2 and ACTN3), ACTN2 being present in all fiber types, whereas ACTN3 is present only in type II fibers, especially type IIx fibers [25, 26]. However, the ACTN3 gene undergoes a mutation that results in the exchange of an arginine by a stop codon at position 577 of the protein causing some individuals not to express ACTN3, which is a hereditary deficiency [25]. However, little is known about the effects of acute exposure to hypoxia in relation to genetic differences and therefore it is necessary to investigate in more detail how different genotypes can influence the acclimatization process. Therefore, the aim of this study was to verify the influence of the R577X polymorphism of the ACTN3 gene on the symptoms of the AMS during the process of acclimatization in healthy individuals exposed 4 hours to simulated high altitude (4500 m).

Materials and methods

Participants

This study was carried out in two stages, the first of which was the collection of epithelial cells from the buccal mucosa from 61 volunteers (39 men and 22 women) for the genetic evaluation of the ACTN3 R577X polymorphism, with twenty-one RR (34.4%), thirty-two RX (52.5%) and eight XX (13.1%). In addition, volunteers responded to the International Physical Activity Questionnaire (IPAQ). The second stage was carried out in the premises of Clube Escola of Universidade Federal de São Paulo (UNIFESP). To participate in the study, volunteers had to be physically active, according to the IPAQ and between 17 and 45 years of age and have no history of cardiovascular diseases. Volunteers who were exposed to altitudes above 2500 m up to 6 months prior to the experiment were excluded from the study. The ACTN3 distribution of volunteers participating to the second stage was RR = 7, RX = 8 and XX =8.

Experimental design

After the first stage of the experiment, individuals returned to the laboratory for the experimental procedure inside the normobaric chamber to 4500 m of altitude. In this situation, volunteers were evaluated in five different moments: (1) immediately before entering the chamber, (2) 1 hour of exposure to hypoxia, (3) 2 hours of exposure to hypoxia, (4) 3 hours of exposure to hypoxia and (5) 4 hours exposure to hypoxia. Blood samples were collected at each time point for evaluation of plasma lactate and glucose concentrations; heart rate (HR) and peripheral oxygen saturation (SpO2) were measured at each moment (Fig. 1). All volunteers were evaluated in the morning and received a standard snack after the first analysis before entering the chamber.

Fig. 1
figure 1

Experimental Design. Participants were evaluated at five different times, the first before entering the camera. Heart Rate (HR), glucose, lactate and peripheral oxygen saturation (SpO2) were measured at each moment. Participants presented for the procedure in groups of three, four or five people at a time, randomly among the genotypes

Full size image

DNA EXTRATION

To determine the genotype of the ACTN3 R557X gene, epithelial cells from the buccal mucosa were collected using a swab on the inside of the cheek, for approximately 30 s. The samples were then stored in a cooled thermal box to preserve the material at low temperature (4 °C) until it was transported to the laboratory. This collection method was chosen because it is a quick, low-invasive, painless, low-cost procedure and still provides a quality genomic DNA sample. Genomic DNA was extracted using the Chelex 100 resin following the manufacturer’s recommended protocol [27].

DNA quantification

The quantification of the genomic DNA was performed in a NanoDrop®-ND1000 spectrophotometer. This equipment evaluates the amount of DNA, uses small volumes (1 μL) without the need of dilution and provides parameters to evaluate its purity, regarding the presence of proteins and phenolic compounds [28].

qPCR GENOTYPING

The SNP (single nucleotide polymorphism) analyzes of the ACTN3 gene were made by the qPCR allele discrimination method (quantitative PCR) using the TaqMan® SNP genotyping assays (Applied Biosystems, Foster City, CA) and QuantStudio 5 (Applied Biosystems®). The technique allows the analysis of the two variant alleles of a SNP in a particular segment of DNA. For this experimental approach, TaqMan® assays that have already been functionally tested (ACTN3 rs1815729) were used, because they offer better performance and more affordable cost compared to the customization of the tests [29].

NORMOBARIC altitude chamber

The Altitude Simulator (CAT - Colorado Altitude Training™/12 CAT-Air Unit) simulates up to 4500 m. This equipment has two air units installed outside the chamber, which performs the gas exchange, increasing nitrogen and reducing O2. This gas exchange generates difference in the O2 concentration inside the chamber that is visible in a display that shows the simulated altitude in real time, measured by a module that contains an O2 cell sensitive to its variations.

AMS symptoms

In order to diagnose AMS, we used a self-assessment questionnaire called the Lake Louise Score [21, 30], which consists in punctuating from zero to three (0 = absence, 1 = mild, 2 = moderate; 3 = severe) the presence of four symptoms (headache, nausea or lack of appetite, fatigue or weakness, dizziness). For the diagnosis it is necessary that the individual is above 2500 m and has the presence of at least two symptoms (totalizing at least 3 points), one being a headache (with at least 1 point) [17, 30].

Physiological variables

Peripheral oxygen saturation (SpO2) was monitored using a Fingerpulse® finger oximeter, model MD300C202, using a dual light source (a red LED and a red infrared LED) and a photodetector that detects variations in light absorption in the arterial blood, since other tissues such as bones and venous vessels normally absorb a constant amount of light over time. The wavelength of the LED is 660 nm and the infrared LED is 905/880 nm with a maximum optical output power of 4 mW. Heart rate (HR) was measured using the same Fingerpulse® finger oximeter model MD300C202 since the use of this type of appliance at rest is adequate [31]. The glucose and lactate evaluations were performed through blood sample collected from the tips of the fingers. At each collection the fingertips were sanitized with BIOSOMA alcohol swab and later drilled with a BIOLAND Model SB-323 auto lancet. The quantification of these variables was performed through reflectance photometry through ROCHE’s Accutrend Plus Monitor, a portable analyzer for the quantitative determination of glucose, lactate, cholesterol and triglycerides [32].

Statistics analysis

To analyze the association between the physiological variables and our study variable (presence of AMS symptoms), we used a Generalized Estimating Equations (GEE) with Tweedie distribution, in which the values of each time were condensed into a single variable counting 114 values (23 individuals, 5 times, less 1 lactate time which an error occur with the equipment). Tweedie distribution was used to fit our GEE model using the results of Lake Louise Score as our main variable in which the most of responses was zero, since Tweedie distribution fit both discrete and continuous data [33, 34]. The GEE model with Tweedie distribution was used to investigate if a physiological variable or ACTN3 polymorphism was associated with an increased chance to develop AMS. In the GEE with the presence of ACTN3 genotypes and with glucose 3 h and glucose 4 h as covariates, the latter entered only the values of these moments. The level of significance was α ≤ 5%. To perform the tests, the IBM SPSS Statistics 21 software was used.

Results

Acute mountain sickness

During the 4 h of exposure to low oxygen pressure mimicking high altitude, three volunteers presented scores related to AMS. One of these volunteers (RX; IMC = 24.7 kg/m2) presented a score 4 between the moments of 2 to 3 h, however, without presence of headache, which eliminates the diagnosis. Of the other two volunteers who presented symptoms (both RRs), one of them (BMI = 24.8 kg/m2) had score 4 at the moment 3 h and score 6 at the moment 4 h, while the other volunteer (BMI = 21.7 kg/m2) presented score 4 at the moment 4 h. In both cases, one of the symptoms reported was headache, which validates the diagnosis of AMS. There were no differences between them and the other volunteers of this study for age, weight, height or BMI (Table 1).

Table 1 Characteristics of AMS and no-AMS groups
Full size table

In order to verify which physiological variables could explain the presence of AMS symptoms, we performed a GEE in which we used the HR, glucose, lactate and SpO2 variables analyzed during the whole time of exposure to hypoxia (N = 114) and we found only SpO2 association [X2 (1, N = 114) = 3.58, p = 0.05] with the symptoms reported through the Lake Louise questionnaire. This result indicates that there is an inverse relationship between the variables, meaning that the lower the value of SpO2, the higher the probability of the volunteer to report a symptom in the questionnaire (β = − 0.04, 95% CI = − 0.09/− 0, P = 0.05). Figure 2 shows the relationship between the physiological variables and scores of Lake Louise during all exposure time to hypoxia.

Fig. 2
figure 2

Physiological Variables in relation to Lake Louise scores during 4 hours exposure to hypoxia. Relationship between the physiological variables SpO2, glucose and HR with mean Lake Louise scores during the five moments of evaluation. The variable lactate did not enter the graph because it did not show relation to the score or change over time. Values ​​represent the mean of ALL volunteers (n = 23) at each time

Full size image

ACTN3 R577X and AMS

To test our hypothesis of the acclimatization difference of the R577X polymorphism of the ACTN3 gene, we included in the statistical model the ACTN3 and the glucose data after 3 and 4 h of exposure, since it was in these moments that volunteers presented the symptoms of AMS (N = 114). The results are shown in Table 2.

Table 2 Result of GEE analysis for Lake Louise, SpO2, ACTN3 and glucose in the moments of 3 and 4 h
Full size table

The statistical model showed association of AMS symptoms with SpO2 [X2 (1, N = 114) = 5.56, p = 0.01] and ACTN3 [X2 (1, N = 114) = 15.60, p < 0.01]. Considering ACTN3 genotypes, RX (β = 1.78, 95% CI = 0.62/2.95, p < 0.01) and RR (β = 2.08, 95% CI = 1.02/3.13, p < 0.01) are associated with Lake Louise, when controlled by the glucose levels after 3 and 4 h. The data expressing these associations are shown in Table 3.

Table 3 Result of GEE analysis for Lake Louise, SpO2, ACTN3 polymorphisms and glucose in the moments of 3 and 4 h
Full size table

Oxygen saturation

In order to investigate which physiological variables could explain the decrease in SpO2 over time, we correlated HR, lactate and glucose to verify their behavior against SpO2 but did not find any variable that showed significant values.

Discussion

Several studies have demonstrated the influence of low oxygen pressure at high altitude with AMS, however, in our study we expected that no volunteer would develop AMS. The reason for this assumption is that we have controlled most of the risk factors such as obesity [35], physical effort [36] and low glycemia [37], since our volunteers had a meal just before entering the chamber, did not have any physical activity inside and had normal BMI. Furthermore, our volunteers were exposed only for 4 hours at conditions mimicking 4500 m. Even having controlled all those risk factors, almost 10% of our volunteers had AMS. First we evaluated whether AMS was related to a decreased SpO2 since it is the main inducing factor. Interestingly we could demonstrate in our study an association between SpO2 with AMS symptoms which corroborates a three-month study in 506 mountaineers of different nationalities in the Alps between 2200 m and 3817 m, that applied the Lake Louise questionnaire and measured SaO2 and HR and demonstrated that the main symptom of AMS, headache, is directly related to low SaO2, in addition to high perception of effort and low water consumption [18]. Our results reinforce this interaction, since among all the physiological variables analyzed only SpO2 was associated with the symptoms reported by the volunteers.

We also analyzed the probability of ACTN3 being a risk factor to AMS, since both of our volunteers that developed the symptoms had the same genotype (RR). Therefore, the association between the ACTN3 R577X polymorphism with the presence of AMS symptoms is the main result of our study. Although several genes have been studied in an attempt to find a relationship with the symptoms [22, 38, 39], our study is the first to correlate this condition to ACTN3 R577X genotypes, showing that individuals with at least one R allele are more susceptible to negative effects of hypoxia during acclimatization. This result, to a certain extent, corroborates previous findings that showed a higher prevalence of X allele in professional mountaineers when compared to normal individuals [40]; higher percentage of type I fibers in populations living in high altitudes such as Quechua of Peru [24] and the Sherpas and Tibetans [23] and in individuals with higher number of capillaries per muscle cross-sectional area [41], which could facilitate the supply of oxygen to the active muscles. Individuals expressing ACTN3 have at least one R allele, and homozygous (RR) individuals have a higher number of type IIx fibers than individuals with the XX genotype, with more type I fibers [42]. In addition, according to Hoppeler and Vogt [43], exposure to hypoxia causes a decrease of about 30% in mitochondrial density without a decrease in capillary density, which would lead to an increase in the supply of oxygen to mitochondria, favoring aerobic metabolism.

The association demonstrated in our study between the symptoms of AMS and ACTN3 genotypes is related to the decrease of SpO2, which is mainly responsible for the presence of AMS symptoms, with headache as the main symptom [18, 19]. This data reinforces our hypothesis that the XX genotype of ACTN3 may have a faster acclimatization in situations of extreme altitude.

Conclusion

We conclude that there may be an association between the R allele of the ACTN3 R577X gene polymorphism and the susceptibility to develop the symptoms of AMS caused by a decreased SpO2.

Limitations of the study

Due to the fact that our study involves genetic variants, the number of analyzed volunteers may not be significant to show a definitive association. Furthermore, in a normobaric chamber, where adverse situations such as cold, wind and physical activity involved in the process of climbing at high altitudes are not present, the effects presented here may have been suppressed. Therefore, based on these restrictions, we suggest that new studies should be performed by other groups in hypobaric hypoxia in order to confirm the findings of this study.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ACTN2:

Alpha actinin 2

ACTN3:

Alpha actinin 3

ACTN3 :

Alpha actinin 3 gene

AMS:

Acute Mountain Sickness

BMI:

Body Mass Index

GEE:

Generalized Estimating Eqs.

HR:

Heart Rate

iPAQ:

International Physical Activity Questionnaire

SaO2 :

Arterial Oxygen Saturation

SpO2 :

Peripherical Oxygen Saturation

References

  1. Kenney WL, Wilmore JH, Costill DL. Exercício na Altitude. Fisiologia do Esporte e do Exercício. Barueri: Editora Manole; 2013.

    Google Scholar 

  2. Hodkinson PD. Acute Exposure to Altitude. J R Army Med Corps. 2011;157(1):85–91.

    Article  CAS  PubMed  Google Scholar 

  3. Ni Q, Wan RQ, Jing YH, Dong XY, Zhang YC. Effect of Acute and Chronic Exposure to High Altitude on the Aerobic and Anaerobic Metabolism in Rats. Anal Cell Pathol. 2015. ID 159549.

  4. Rahn H, Otis AB. Man’s respiratory response during and after acclimatization to high altitude. Am J Phys. 1949;157(3):445–62.

    Article  CAS  Google Scholar 

  5. Bärtsch P, Swenson ER. Acute high-altitude illness. N Engl J Med. 2013;368(24):2294–302.

    Article  PubMed  CAS  Google Scholar 

  6. Lipsitz LA, Hashimoto F, Lubowsky LP, Mietus J, Moody GB, Appenzeller O, Goldberger AL. Heart rate and respiratory rhythm dynamics on ascent to high altitude. Br Heart J. 1995;74(4):390–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Brooks GA, Butterfield GE, Wolfe RR, Groves BM, Mazzeo RS, Sutton JR, Wolfel EE, Reeves JT. Increased dependence on blood glucose after acclimatization to 4,300 m. J Appl Physiol. 1991;70(2):919–27.

    Article  CAS  PubMed  Google Scholar 

  8. Brooks GA, Wolfel EE, Groves BM, Bender PR, Butterfield GE, Cymerman A, Mazzeo RS, Sutton JR, Wolfe RR, Reeves JT. Muscle accounts for glucose disposal but not blood lactate appearance during exercise after acclimatization to 4,300 m. J Appl Physiol. 1992;72(6):2435–45.

    Article  CAS  PubMed  Google Scholar 

  9. Ge RL, Simonson TS, Gordeuk V, Prchal JT, McClain DA. Metabolic aspects of high-altitude adaptation in Tibetans. Exp Physiol. 2015;100(11):1247–55.

    Article  CAS  PubMed  Google Scholar 

  10. West J. The physiologic basis of high-altitude diseases. Ann Intern Med. 2004;41(10):789–800.

    Article  Google Scholar 

  11. Weil JV. Ventilatory control at high altitude. In: Cherniack NS, Widdicome JG, editors. Handbook of physiology. eds ed. Bethesda: American Physiologic Society; 1986. p. 703–27.

    Google Scholar 

  12. Heistad DD, Abboud FM, Dickinson W. Richards lecture: circulatory adjustments to hypoxia. Circulation. 1980;61(3):463–70.

    Article  CAS  PubMed  Google Scholar 

  13. Bärtsch P, Gibbs SR. Effect of altitude on the heart and the lungs. Circulation. 2007;116(19):2191–202.

    Article  PubMed  Google Scholar 

  14. West J. Health considerations for managing work at high altitude. In: STELLMAN JM, Encyclopaedia of occupational health and safety. Geneva: International Labour Organization; 1998.

    Google Scholar 

  15. Shah NM, Windsor JS, Meijer H, Hillebrandt D, Are UK. Commercial expeditions complying with wilderness medical society guidelines on ascent rates to altitude? J Travel Med. 2011;18:214–6.

    Article  PubMed  Google Scholar 

  16. Vardy J, Vardy J, Judge K. Acute Mountain sickness and ascent rates in trekkers above 2500 m in the Nepali Himalaya. Aviat Space Environ Med. 2006;77(7):742–4.

    PubMed  Google Scholar 

  17. Carod-Artal FJ. High-altitude headache and acute mountain sickness. Neurologia. 2014;29(9):533–40.

    Article  CAS  PubMed  Google Scholar 

  18. Burtscher M, Mairer K, Wille M, Broessner G. Risk factors for high-altitude headache in mountaineers. Cephalalgia. 2011;31(6):706–11.

    Article  PubMed  Google Scholar 

  19. Karinen HM, Peltonen JE, Kähönen M, Tikkanen HO. Prediction of Acute Mountain sickness by monitoring arterial oxygen saturation during ascent. High Alt Med Biol. 2010;11(4):325–32.

    Article  PubMed  Google Scholar 

  20. Hsu TY, Weng YM, Chiu YH, Li WC, Chen PY, Wang SH, Huang KF, Kao WF, Chiu TF, Chen JC. Rate of ascent and Acute Mountain sickness at high altitude. Clin J Sport Med. 2014;25(2):95–104.

    Article  Google Scholar 

  21. Roach RC, Bärtsch P, Oelz O, Hackett P. Lake Louise AMS Scoring Consensus Committee: The Lake Louise acute mountain sickness scoring system. In: Hypoxia and Molecular Medicine. JR Sutton, C Houston, G Coates, eds. Queen City printers, Burlington, VT. 1993. pp. 272–274.

    Google Scholar 

  22. MacInnis MJ, Koehle MS, Rupert JL. Evidence for a genetic basis for altitude illness: 2010 update. High Alt Med Biol. 2010;11(4):349–68.

    Article  CAS  PubMed  Google Scholar 

  23. Gilbert-Kawai ET, Milledge JS, Grocott MP, Martin DS. King of the mountains: Tibetan and Sherpa physiological adaptations for life at high altitude. Physiology (Bethesda). 2014;29(6):388–402.

    CAS  Google Scholar 

  24. Rosser BW, Hochachka PW. Metabolic capacity of muscle fibers from high-altitude natives. Eur J Appl Occup Physiol. 1993;67(6):513–7.

    Article  CAS  Google Scholar 

  25. North K, Beggs AH. Deficiency of a skeletal muscle isoform of a-actinin (α-actinin-3) in merosin-positive congenital muscular dystrophy. Neuromuscul Disord. 1996;6(4):229–35.

    Article  CAS  PubMed  Google Scholar 

  26. North KN, Yang N, Wattanasirichaigoon D, Mills M, Easteal S, Beggs AH. A common nonsense mutation results in alpha-actinin-3 deficiency in the general population. Nat Genet. 1999;21(4):353–4.

    Article  CAS  PubMed  Google Scholar 

  27. Walsh PS, Metzger DA, Higushi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 2013;54(3):134–9.

    Article  CAS  PubMed  Google Scholar 

  28. Desjardins P, Conklin D. NanoDrop microvolume quantitation of nucleic acids. J Vis Exp. 2010;22(45):2565.

    Google Scholar 

  29. Schadock I, Schneider A, Silva ED, Buchweitz MR, Correa MN, Pesquero JB, Paredes-Gamero EJ, Araujo RC, Barros CC. Simple method to genotype the ACTN3 r577x polymorphism. Genet Test Mel Biomarkers. 2015;19(5):253–7.

    Article  CAS  Google Scholar 

  30. Roach RC, Hackett PH, Oelz O, Bärtsch P, Lucks AM, MacInnis MJ, Bailie JK, The Lake Louise AMS Score Consensus Committee. The. Lake Louise Acute Mountain sickness score. High Alt Med Biol 2018. 2018;19(1):4–6.

    Article  Google Scholar 

  31. Iyriboz Y, Powers S, Morrow J, Ayers D, Landry G. Accuracy of pulse oximeters in estimating heart rate at rest and during exercise. Br J Sports Med. 1991;25(3):162–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Baldari C, Bonavolontà V, Emerenziani GP, Gallotta MC, Silva AJ. Guidetti L accuracy, reliability, linearity of Accutrend and lactate pro versus EBIO plus analyzer. Eur J Appl Physiol. 2009;107(1):105–11.

    Article  PubMed  Google Scholar 

  33. Dunn PK. Occurrence and quantity of precipitation can be modelled simultaneously. Int J Climatol. 2004;24:1231–9.

    Article  Google Scholar 

  34. Swan T. Generalized Estimating Equations When the Response Variable Has a Tweedie Distribution: An Application for Multi-Site Rainfall Modelling. Honours thesis. University of Southern Queensland, Department of Mathematics and Computing; 2006.

    Google Scholar 

  35. SanMartin R, Brito J, Siques P, Léon-Velarde F. Obesity as a conditioning factor for high-altitude diseases. Obes Facts. 2017;10:363–72.

    Article  CAS  Google Scholar 

  36. Rupp T, Jubeau M, Millet GY, Perrey S, Esteve F, Wuyan B, Levy P, Verges S. The effect of hypoxemia and exercise on acute mountain sickness symptoms. J Appl Physiol. 2013;114:180–5.

    Article  PubMed  Google Scholar 

  37. Kelly KR, Williamson DL, Fearly CE, Kriz DA, Krishnan RK, Huang H, Ahn J, Loomis JL, Kirwan JP. Acute altitude-illness hypoxia suppresses plasma glucose and leptin in healthy humans. Metabolism. 2010;59:200–5.

    Article  CAS  PubMed  Google Scholar 

  38. Tomar A, Malhotra S, Sarkar S. Polymorphism profiling of nine high altitude relevant candidate gene loci in acclimatized sojourners and adapted natives. BMC Genet. 2015;16:112.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  39. MacInnis MJ, Koehle MS. Evidence for ans against genetic predispositions to acute and chronic altitude illness. High Alt Med Biol. 2016;17(4):281–93.

    Article  PubMed  Google Scholar 

  40. Djarova T, Bardarev D, Boyanov D, Kaneva R, Atanasov P. Performance enhancing genetic variants, oxygen uptake, heart rate, blood pressure and body mass index of elite high altitude mountaineers. Acta Physiol Hung. 2013;100(3):289–301.

    Article  CAS  PubMed  Google Scholar 

  41. Kayser B, Hoppeler H, Desplanches D, Marconi C, Broers B, Cerretelli P. Muscle ultrastructure and biochemistry of lowland Tibetans. J Appl Physiol. 1996;81(1):419–25.

    Article  CAS  PubMed  Google Scholar 

  42. Vincent B, De Bock K, Ramaekers M, den Eede EV, Van Leemputte M, Hespel P, Thomis MA. ACTN3 (R577X) genotype is associated with fiber type distribution. Physiol Genomics. 2007;32:58–63.

    Article  CAS  PubMed  Google Scholar 

  43. Hoppeler H, Vogt M. Muscle tissue adaptations to hypoxia. J Exp Biol. 2001;204(Pt 18):3133–9 Review.

    CAS  PubMed  Google Scholar 

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Acknowledgements

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Funding

This paper is part of a master’s degree dissertation, which was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES), Associação Fundo de Incentivo à Pesquisa (AFIP) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2014/27198–8).

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Authors and Affiliations

  1. Department of Psychobiology, UNIFESP, Botucatu Street, 862, First Floor, Vila Clementino, SP, ZIP, São Paulo, 04023062, Brazil

    Ricardo Muller Bottura & Debora Cristina Hipolide

  2. Department of Biophysics, UNIFESP, São Paulo, Brazil

    Giscard Humberto Oliveira Lima & João Bosco Pesquero

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  1. Ricardo Muller Bottura
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  2. Giscard Humberto Oliveira Lima
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Contributions

RMB collected and analyzed the data during the experiment, performed statistical analysis and wrote the manuscript. DCH also analyzed the data during the experiment. GHOL and JBP were responsible for the genotyping of volunteers and correction of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ricardo Muller Bottura.

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All volunteers signed a Free and Informed Consent Term (TCLE) and the study was approved by the Research Ethics Committee of the Federal University of São Paulo under number 2,001,055.

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Bottura, R.M., Lima, G.H.O., Hipolide, D.C. et al. Association between ACTN3 and acute mountain sickness. Genes and Environ 41, 18 (2019). https://doi.org/10.1186/s41021-019-0133-8

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