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Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis

Genes and Environment volume 43, Article number: 49 (2021) Cite this article

Abstract

Background

Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6A is a highly conserved modification across the species.

Results

The results of m6A sequences showed that m6A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs.

Conclusions

These findings suggested that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6A modifications in amphibian species.

Introduction

RNA modifications play crucial roles in gene expression [1]. As the most universal form of post-transcriptional RNA modifications, N6-methyladenosine (m6A) modification has become a new research area in epigenetic [2]. Recent studies have shown that the m6A modification modulates the function of the RNA molecule in multiple ways through its novel functions [3, 4]. The regulatory association between RNA m6A modification and spermatogenic function has been discovered [5]. Cumulative studies have found that knockout of RNA m6A regulators in the testis leads to abnormal metabolism of the target RNAs, which eventually causes spermatogenetic disorders and infertility [6]. The m6A modification is found in different species of RNA, including tRNA, mRNA, rRNA, and long non-coding RNAs (lncRNAs) [7, 8]. In addition, the m6A modification can affects many properties of RNA, including gene translation [9], splicing [10], and long non-coding RNA-mediated gene silencing [11].

LncRNAs are non-coding RNAs comprising more than 200 nucleotides without protein coding function and engaging in diverse biological processes across every branch of life [12]. Increasing evidence indicetates that lncRNAs play an important role in regulating multiple processes of gene expression [13]. Studies have also found that regulation of lncRNAs can affect mRNA transcription, splicing, translation and stability [14]. It has been widely recognized that dysregulated lncRNAs play an important part in many diseases [15]. In recent years, m6A modification of lncRNAs gains great attention and this modification has shown to control mammalian gene expression [16]. To our knowledge, the profile of m6A modification of lncRNAs in amphibians remain to be explored.

Atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine, AZ) is an EDCs used extensively as an herbicide worldwide [17, 18]. AZ has been reported that it can cause endocrine disruption in mammals, birds, reptiles, and amphibians by affecting normal reproductive function and development in these organisms [19, 20]. Xenopus laevis (X. laevis) is a kind of amphibian widely used as an ideal model organism for testing EDCs exposure [21]. Recently, AZ has been shown to cause demasculinization and complete feminization in male X. laevis [22]. In our previous studies, we investigated biological response of X. laevis exposed to AZ (0.1, 1, 10 or 100 μg/L) for 90 days in the water environment. We found that AZ induced the reduction of gonad weight and gonado somatic index of male X. laevis. Meanwhile, AZ induced histological changes in testes of the frogs from all of AZ treatments including irregular shape of seminiferous lobules and large empty spaces [23]. However, the mechanism of AZ-induced abnormal development of male X. laevis is unclear. Therefore, it is necessary to explore the potential changes of m6A modification of lncRNAs which maybe play an important role in the abnormal testis of male AZ-exposed X. laevis.

In general, mRNA m6A is enriched around the stop codon and 3′ UTR in mammals, hypothesized to contribute towards the control of transcript stability and translation [24]. However, in our previous results, the m6A peak observed clearly enriched in the start codon and stop codon in X. laevis [25]. In contrast to mRNAs, m6A residues in lncRNAs are distributed along the whole body of transcripts and are more concentrated in the lncRNAs undergoing alternative splicing. Furthermore, Dominissini et al. also identified that m6A in exonic regions was preferentially found in longer exons of 400 nucleotides or more [26]. LncRNAs are defined as seven types of transcripts such as including included lincRNA, antisense, processed transcript, sense intronic, 3 prime overlapping ncRNA, sense overlapping, and macro lncRNA [27].

Here, we first analysed the profile of m6A modification of lncRNAs in X. laevis and dysregulated m6A methylation of lncRNAs in the AZ-exposed male X. laevis. Then, we predicted classification function and involved signaling pathways of dysregulated m6A methylation of lncRNAs in AZ-exposed male X. laevis. Our data will provide the basis for future studies of m6A methylation of lncRNAs about function and biological significance in amphibians and the insightful information of the abnormal testis development in AZ-exposed male X. laevis.

Methods

Ethic approval

All animal experiments were performed in accordance with relevant guidelines and regulations. All experiments were complied with the “Principles of Animal Care”. The protocol was assessed and approved by the Committee on the Ethics of Animal Experiments of Shandong Academy of Occupational Health and Occupational Medicine.

Sample animals

The adult male and female X. laevis were purchased from the Chinese Academy of Sciences (Beijing, China) and natural mated to produce offspring. UV-treated and carbon-filtered laboratory freshwater was used for the acclimatization of frogs in the laboratory and for all subsequent exposures. The X. laevis were kept at an average water temperature of 22 ± 2 °C at pH 7.5, under 12 h light and 12 h dark cycle. Tadpoles were fed fairy shrimp (Artemia nauplii) eggs in a young age daily and pork liver three times per week ad libitum when the tadpoles completed metamorphosis.

At Nieuwkoop-Faber (NF) stage 47 (13 d post-hatch), mixed sex tadpoles (n = 320) from one adult pairing were randomly divided into two groups. AZ (purity of 97%) obtained from Sigma (Chemical Co., USA) dissolved in solvent vehicle DMSO (0.01%). The tadpoles were exposed to AZ at dosages of 100 μg/L for 180 days. The control tadpoles were treated with 0.01% DMSO only. Test solutions were refreshed by 50% replacement every 48 h. Animals were observed daily for monitoring morphological changes and health status [23]. The X. laevis were sacrificed after being exposed to AZ for 180 days. The exposure time is based on the developmental characters of male X. laevis. The testis tissues were collected and weight, and then stored at − 80 °C immediately for further analysis.

LncRNAs preparation

For each group, at least three biological replicates were run [28]. Three testes from controls and three ones from 100 μg/L AZ-treated groups were selected randomly for lncRNAs analysis. Then, total RNA of tissue was extracted using TRIzol reagent (Invitrogen Corporation, CA, USA). The concentration and purity of RNA were evaluated by NanoDrop® ND-2000 spectrometer (Thermo, Waltham, MA, USA). The integrity of RNA was determined by denaturing gel electrophoresis. RNA samples were further purified and converted to double-stranded cDNA for microarray analysis which was conducted according to Agilent® Xenopus 4 × 44 K Gene Expression Microarrays protocols.

lncRNAs m6A MeRIP sequencing

M6A of lncRNAs was sequenced by MeRIP sequencing using the latest Illumina HiSeq sequencer. Briefly, fragmented RNA was incubated with anti-m6 A polyclonal antibody (Synaptic Systems, 202,003) in IPP buffer for 2 h at 4 °C and the mixture was immune precipitated. Then, bound RNA was eluted from the beads in buffer and then extracted by following the manufacturer’s instruction. Both the input sample without immune precipitation and the m6A IP samples were subjected to 150 bp paired-end sequencing on Illumina HiSeq sequencer. Paired-end reads were harvested and were quality controlled by Q30. Detailed methods were described in our previous study [25].

Data analysis

After sequencing, quality control of the paired-end reads was performed with Q30, which was subjected to 3’adaptor trimming and low quality reads removing to generate clean reads by Cutadapt software (v1.9.3). Firstly, clean reads of all libraries were aligned to reference genome using bowtie 2 [29] software and mapped to genome by hisat 2 software (v2.04) [30]. Methylated sites on lncRNAs (methylated sites with a score (− 10*log10, P-value) of > 3) were identified by MACS software. Differentially m6A-methylated sites on lncRNAs were detected by diffReps and the identified sites overlapping with exons of lncRNAs were chosen for further analysis. Pathway enrichment analysis was used to maps genes to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.

Results

Overview of m6A sites within lncRNAs in the testes of control and AZ-exposed X. laevis

MeRIP-seq analysis of lncRNAs derived from the testes of X. laevis revealed that there were 1298 m6A peaks among 908 lncRNAs in control group. While 1501 m6A peaks among 1055 lncRNAs were detected in the testes of AZ exposed X. laevis. Importantly, 1100 m6A recurrent sites were consistently detected in controls and AZ-exposed groups (Fig. 1).

Fig. 1
figure 1

Venn diagram showing the overlap of m6A peaks within lncRNAs in AZ-exposed and control groups

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Distribution profiles of m6A-methylated lncRNAs

To further analyze the distribution profiles of m6A-methylated lncRNAs, these distribution positions of modified lncRNAs were categorized into 6 groups: bidirectional, exon sense-overlapping, intergenic, intron sense-overlapping, intron sense-overlapping, natural antisense. Particularly, we found that the most m6A-methylated lncRNAs are highly enriched in intergenic region (67.1% in control and 67.8% in AZ-exposed groups) (Fig. 2a and b). In the controls, the distribution positions of m6A-methylated lncRNAs had a highly fold enrichment in bidirectional (95.24%), exon sense-overlapping (95.74%) and intergenic regions (96.15%). Meanwhile, in AZ-exposed groups, the distribution positions of m6A-methylated lncRNAs had a highly fold enrichment in intergenic (86.65%), intronic antisense (80.47%) and bidirectional (76.88%) (Fig. 2c and d).

Fig. 2
figure 2

Overview the distribution positions of m6A-methylated lncRNAs in the testes of X. laevis from control and AZ-exposed groups. a: Pie charts showing the percentage of the distribution positions of m6A-methylated lncRNAs relatived to its mRNA in control group. b: Pie charts showing the percentage of the distribution positions of m6A-methylated lncRNAs in AZ-exposed group. c: Distributions of mean fold enrichment of m6A-methylated lncRNAs in six segments in control group. d: Distributions of mean fold enrichment of m6A-methylated lncRNAs in six segments in AZ-exposed group. Error bars represent the standard error of the mean. The mean fold enrichment in the intergenic segments was the largest both in the control and AZ-exposed group with lower standard error of the mean

Full size image

Differentially m6A modification sites of lncRNAs in X. laevis exposed to 100 μg/L AZ

The results showed that 198 differentially methylated m6A sites were detected among 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites (Table S1). The top ten up- and down-methylated m6A sites of lncRNAs with the highest fold change (FC) values were shown in Tables 1 and 2.

Table 1 The top ten up-methylated m6A sites of lncRNAs
Full size table
Table 2 The top ten down-methylated m6A sites of lncRNAs
Full size table

Further analysis showed that according to the positional relationships of lncRNAs near the coding gene transcripts, most differentially methylated lncRNAs were assigned to intergenic (Fig. 3a and b). Besides, among the lncRNAs with up-methylated sites, those within the intergenic had the highest mean of FC. While among the lncRNAs with down-methylated sites, those within the intron sense-overlapping had the highest mean of FC (Fig. 3c).

Fig. 3
figure 3

Distribution of differentially methylated m6A sites of lncRNAs. a: Pie charts showing the percentage of up-methylated m6A peaks in six segments. b: Pie charts showing the percentage of down-methylated m6A peaks in six segments. c: Statistics of meanof the distribution positions of m6A-methylated lncRNAs in six segments with up- and down-methylated sites. Error bars represent the standard error of the mean

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The enrichment pathways of differentially m6A-methylated lncRNAs-associated target genes by KEGG

To further explore the roles of differentially m6A-methylated lncRNAs in the abnormal development of testis from AZ-exposed X. laevis, we performed KEGG pathway analysis of differentially m6A-methylated lncRNAs-related genes to look for the potential key pathways. The result of pathway analysis indicated that 2 pathways with highly enrichment score (−log10 (P-value)) were acquired in up-methylated sequencing data. The two signaling pathways, as “SNARE interactions in vesicular transport and Ubiquitin mediated proteolysis”, were shown in Fig. 4a. Meanwhile, 8 pathways were found in down-regulated sequencing data, including “Terpenoid backbone biosynthesis, GnRH signaling pathway, Cell cycle, AGE-RAGE signaling pathway in diabetic complications, Vascular smooth muscle contraction, Wnt signaling pathway, Autophagy-animal, NOD-like receptor signaling pathway” (Fig. 4b).

Fig. 4
figure 4

The annotated significant pathways targeted by the enrichment score of the differentially m6A-methylated (up-methylated (a) and down-methylated (b)) lncRNAs-related genes in testis of X. laevis exposed to 100 μg/L AZ. The horizontal axis is the -LogP (logarithm of P-value) for the pathway and the vertical axis is the pathway category

Full size image

Discussion

M6A modification is characterized by wide existence, unique distribution, and dynamic reversibility [31, 32]. It has also been found that enhancer RNAs, non-coding transcripts produced from enhancer regions are highly m6A modified [33]. M6A has been shown to be the abundant internal modification in eukaryotic mRNAs [34]. Emerging findings have shed light on the involvement of m6A modification of lncRNAs [35, 36]. A recent study showed that m6A methylation regulatory network regulates RNA processing and participates in various cellular biological processes, such as biological rhythm, immune modulation, fat metabolism, reproductive development [37]. Several studies showed that AZ could disrupt germ cell and Sertoli cell numbers. The X. laevis exposed to AZ has also been shown to reduce testicular volume and testosterone and induce testicular oogenesis [38,39,40]. Our results showed that AZ can elicit reproductive toxicity in developing male X. laevis in our previous study [23]. In addition, m6A, an epitranscriptomic mark regulating gene expression, plays essential roles during spermatogenesis [41]. Till date many potential lncRNAs have been reported in spermatogenesis and male infertility and they were identified to be important factors for spermatogenesis which could also be exploited as markers of male infertility [42]. To explore the m6A modification profile of lncRNAs in the testis of X. laevis and the changes of m6A modification of lncRNAs in AZ-exposed X. laevis, we examined the transcriptome-wide m6A modification of lncRNAs distribution in the testis of AZ-exposed X. laevis. Meanwhile, the changes of m6A modification of lncRNAs distribution were analyzed by exogenous stimulation.

The profiles of m6A modification of lncRNAs in mammals were identified in recent years, such as mouse, rat and human [43,44,45,46]. Meanwhile, large intergenic noncoding RNAs (lincRNAs) which are one class of lncRNAs transcribed from intergenic regions are defined [47].. Key roles for lincRNAs in certain biological processes are starting to emerge. LincRNAs are a novel class of gene regulators that function as signals, scaffolds, molecular decoys and mediators of long-range chromatin interactions [48]. The identification of specific lincRNAs has shown the importance of lincRNAs in developmental biology. For example, Yang et al. found that m6A modification of linc1281 mediated a competing endogenous RNA (ceRNA) model to regulate mouse embryonic stem cells differentiation [49]. In our study, the patterns of the m6A modification of lncRNAs were identified in X. laevis. Our results showed that the m6A-methylated lncRNAs mainly enriched in the intergenic region, namely m6A modification maily enriched in lincRNAs in X. laevis. Additionally, most of differentially expressed m6A modificationed also enriched in lincRNAs in AZ-exposed X. laevis. Our results suggested that m6A modification of lincRNAs may play a significant role in abnormal testis development of amphibian species.

Differentially m6A modification of lncRNAs were identified by comparing AZ-exposed testes of X. laevis to controls. Here, this result revealed a potential role of m6A modification sites of lncRNAs in testes of X. laevis induced by environmental agents such as AZ. Interestingly, we found lncRNA “XR_001933134” was up-regulated in the testis of AZ-treated X. laevis in our previous study, but the m6A modification of which was down-regulated [50]. The result showed that m6A modification may negatively regulate the expression of lncRNA “XR_001933134”. Wu et al. demonstrated that m6A modification of lncRNAs may increase lncRNA RP11 expression [35]. Ban et al. indicated that dysregulation of m6A modification might account for aberrant expression of LNCAROD in HNSCC [51]. Consequently, our results suggested that the negative regulatory relationship between m6A modification of lncRNAs and the expression of lncRNAs in abnormal testis development of X. laevis exposed to 100 μg/L AZ. Meanwhile, Liu et al. found that m6A modification participated in the upregulation of MALAT1 in renal fibrosis and m6A modification of lncRNA MALAT1 can increase its RNA stability in mammal [52]. Therefore, we predicted that m6A modification of lncRNAs may regulate their expression which involved in abnormal testis development of AZ-exposed X. laevis.

Up to now, no data has been reported about the pathway analysis of m6A-methylated lncRNA-associated target genes in AZ-treated X. laevis. Therefore, in the present study, we used KEGG pathway annotation method to analyze the m6A-methylated lncRNA-associated target genes in the testes of X. laevis exposed to100 μg/L AZ. The results of KEGG pathway analysis indicated that 10 pathways were involved in the current sequencing data.

In current study, the top one term was “SNARE interactions in vesicular transport” signaling pathway. SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins could drive vesicle fusion between endosomal compartments in eukaryotic cells [53]. SNARE proteins establish the core membrane fusion machinery of intracellular transport and intercellular communication, which contribute to cell growth, cell expansion, pathogen defense and homeostasis [54, 55]. Additionally, acrosome assembly in spermatogenesis and acrosome reaction in the interaction between sperm and oocyte are unique processes of vesicle synthesis, transportation and fusion, which are the basis of sperm fertilization [56, 57]. The previous study has also shown that SNARE syntaxin was associated with the acrosome in spermatids during sperm development in the testis [58]. Hence, we predicted that m6A-methylated lncRNAs included in SNARE interactions in vesicular transport signaling pathway may play an important role in the abnormal testis tissues of AZ-exposed X. laevis.

It is known that ubiquitin mediated proteolysis possesses many biological processes in controlling cell signaling, regulating cell proliferation, apoptosis, and immune responses [59, 60]. Ubiquitination is also a kind of the versatile cellular regulatory mechanisms and ubiquitin binds to protein playing a crucial role in substrate specificity [61]. In particular, several evidences have demonstrated that X. laevis offers the ability to generate soluble proteins, which capable to carry out the biochemical modifications of protein ubiquitylation [62]. Ubiquitylation usually occurs lysine residues and the residues could bond with ubmolecules and then target proteins for destruction [63]. Moreover, ubiquitin is highly expressed in mammalian gametes and embryos at any particular stage of development and ubiquitin ligasesare very active in the testis [64]. However, the study on ubiquitin mediated of gametogenesis in amphibian is sketchy. Our present study indicated that “ubiquitin mediated proteolysis pathway” regulated by m6A-methylated lncRNAs may involve in the abnormal testis development of X. laevis exposed to AZ.

Gonadotropin-releasing hormone (GnRH) was synthesized in hypothalamic neurons and binded to specific G-protein coupled receptors on the gonadotrope cell surface. It could regulate the biosynthesis and secretion of gonadotropin such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH) which are required for the testis to produce bothmature sperm [65, 66]. Recent reports have shown that active immunization against GnRH could inhibit synthesis or secretion of gonadotropins, and thereby induced the termination of gametogenesis, inhibited reproductive behavior, and finally caused infertility of both male and female animals [67]. Moreover, orexin receptors type 1 (OX1R) was G protein-coupled receptors whose receptor expression was found in the pituitary of X. laevis [68]. The expression level was regulated by gonadal GnRH [65]. Therefore, m6A-methylated lncRNAs involved in “GnRH signaling pathway”may play an important role in damaged testis of AZ-exposed X. laevis.

Interestingly, “SNARE interactions in vesicular transport”, “NOD-like receptor signaling pathway” and “GnRH signaling pathway” were also found in the results of KEGG of differentially m6A-methylated mRNAs in X. laevis exposed to 100 μg/L AZ in our previous study [25]. The results showed that 3 mutual pathways may play important regulatory roles and possibly induce testes damage in AZ-exposed X. laevis.

Conclusion

We examined the m6A modification profile of lncRNAs in testis tissues of X. laevis with and without treatment with 100 μg/L AZ through m6A sequencing analysis using the latest Illumina HiSeq sequencer. The results indicated that AZ leaded to alter expression profile in 198 m6A modification sites of lncRNAs (89 up-methylated and 109 down-methylated) which mainly enriched in lincRNAs. KEGG pathway analysis indicated that the “SNARE interactions in vesicular transport”, “GnRH signaling pathway”and “NOD-like receptor signaling pathway” may be closely associated with abnormal testis development of X. laevis due to exposure to AZ. Analysis results showed a negative correlation between m6A modification of lncRNA and lncRNA abundance, suggesting a regulatory role of m6A of lncRNAs in amphibious gene expression. Our study provides a fundamental contribution to possible molecular mechanisms underlying the reproductive system toxicity of AZ on male X. laevis.

However, in our study the first m6A transcriptome-wide map of lncRNAs of an amphibian species X. laevis presented here provides a starting roadmap for uncovering the role of m6A modification of lncRNAs that may affect/control amphibian testis development in the future. Meanwhile, our study characterized the differential m6A methylome of lncRNAs in the testis of X. laevis exposed to 100 μg/L AZ relative to the controls. The results suggested a possible association between m6A methylation and the regulation of developmental metabolism in the testis of X. laevis exposed to 100 μg/L AZ. So these results may provide a fundamental contribution to future studies aimed to gain deeper insights.

Availability of data and materials

All data generated and or analyzed during this study are included in this published article and its supplementary information files.

Abbreviations

M6A:

N6-methyladenosine

X. laevis :

Xenopus laevis

AZ:

Atrazine

LncRNAs:

Long non-coding RNAs

EDCs:

Endocrine disrupting chemicals

FC:

Fold change

KEGG:

Kyoto Encyclopedia of Genes and Genomes

LincRNAs:

Large intergenic noncoding RNAs

ceRNA:

Competing endogenous RNA

SNARE:

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor

GnRH:

GONADOTROPIN-releasing hormone

FSH:

Follicle-stimulating hormone

LH:

Luteinizing hormone

OX1R:

Orexin receptors type 1

References

  1. Meyer KD, Jaffrey SR. Rethinking m (6) a readers, writers, and erasers. Annu Rev Cell Dev Biol. 2017;33(1):319–42. https://doi.org/10.1146/annurev-cellbio-100616-060758.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Niu Y, Zhao X, Wu YS, Li MM, Wang XJ, Yang YG. N6-methyl-adenosine (m6A) in RNA: an old modification with a novel epigenetic function. Genomics Proteomics Bioinformatics. 2013;11(1):8–17. https://doi.org/10.1016/j.gpb.2012.12.002.

    Article  CAS  PubMed  Google Scholar 

  3. Li HB, Tong J, Zhu S, Batista PJ, Duffy EE, Zhao J, et al. M (6) a mRNA methylation controls T cell homeostasis by targeting the IL-7/STAT5/SOCS pathways. Nature. 2017;548(7667):338–42. https://doi.org/10.1038/nature23450.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Zhang C, Chen Y, Sun B, Wang L, Yang Y, Ma D, et al. M (6) a modulates haematopoietic stem and progenitor cell specification. Nature. 2017;549(7671):273–6. https://doi.org/10.1038/nature23883.

    Article  CAS  PubMed  Google Scholar 

  5. Zhao TX, Wang JK, Shen LJ, Long CL, Liu B, Wei Y, et al. Increased m6A RNA modification is related to the inhibition of the Nrf2-mediated antioxidant response in di-(2-ethylhexyl) phthalate-induced prepubertal testicular injury. Environ Pollut. 2020;259:113911. https://doi.org/10.1016/j.envpol.2020.113911.

    Article  CAS  PubMed  Google Scholar 

  6. Cai Z, Niu Y, Li H. RNA N6-methyladenosine modification, spermatogenesis, and human male infertility. Mol Hum Reprod. 2021;27(6). https://doi.org/10.1093/molehr/gaab020.

  7. Engel M, Eggert C, Kaplick PM, et al. The Role of m (6) A/m-RNA Methylation in Stress Response Regulation. Neuron. 2018;99(2)):389–403.e9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Nagarajan A, Janostiak R, Wajapeyee N. Dot blot analysis for measuring global N (6)-Methyladenosine modification of RNA. Methods Mol Biol. 2019;1870:263–71. https://doi.org/10.1007/978-1-4939-8808-2_20.

    Article  CAS  PubMed  Google Scholar 

  9. Meyer KD, Patil DP, Zhou J, Zinoviev A, Skabkin MA, Elemento O, et al. 5′ UTR m (6) a promotes cap-independent translation. Cell. 2015;163(4):999–1010. https://doi.org/10.1016/j.cell.2015.10.012.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Xiao W, Adhikari S, Dahal U, Chen YS, Hao YJ, Sun BF, et al. Nuclear m (6) a reader YTHDC1 regulates mRNA splicing. Mol Cell. 2016;61(4):507–19. https://doi.org/10.1016/j.molcel.2016.01.012.

    Article  CAS  PubMed  Google Scholar 

  11. He Y, Hu H, Wang Y, Yuan H, Lu Z, Wu P, et al. ALKBH5 inhibits pancreatic Cancer motility by decreasing long non-coding RNA KCNK15-AS1 methylation. Cell Physiol Biochem. 2018;48(2):838–46. https://doi.org/10.1159/000491915.

    Article  CAS  PubMed  Google Scholar 

  12. Moran VA, Perera RJ, Khalil AM. Emerging functional and mechanistic paradigms of mammalian long non-coding RNAs. Nucleic Acids Res. 2012;40(14):6391–400. https://doi.org/10.1093/nar/gks296.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Bhan A, Mandal SS. Long noncoding RNAs: emerging stars in gene regulation, epigenetics and human disease. ChemMedChem. 2014;9(9):1932–56. https://doi.org/10.1002/cmdc.201300534.

    Article  CAS  PubMed  Google Scholar 

  14. Kornienko AE, Guenzl PM, Barlow DP, Pauler FM. Gene regulation by the act of long non-coding RNA transcription. BMC Biol. 2013;11(1):59. https://doi.org/10.1186/1741-7007-11-59.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Liu X, Li Y, Wen J, Qi T, Wang Y. Long non-coding RNA TTN-AS1 promotes tumorigenesis of ovarian cancer through modulating the miR-139-5p/ROCK2 axis. Biomed Pharmacother. 2020;125:109882. https://doi.org/10.1016/j.biopha.2020.109882.

    Article  CAS  PubMed  Google Scholar 

  16. Coker H, Wei G, Brockdorff N. m6A modification of non-coding RNA and the control of mammalian gene expression. Biochim Biophys Acta Gene Regul Mech. 2019;1862(3):310–8. https://doi.org/10.1016/j.bbagrm.2018.12.002.

    Article  CAS  PubMed  Google Scholar 

  17. Solomon KR, Giesy JP, LaPoint TW, Giddings JM, Richards RP. Ecological risk assessment of atrazine in north American surface waters. Environ Toxicol Chem. 2013;32(1):10–1. https://doi.org/10.1002/etc.2050.

    Article  CAS  PubMed  Google Scholar 

  18. Kong X, Jiang J, Ma J, Yang Y, Liu W, Liu Y. Degradation of atrazine by UV/chlorine: efficiency, influencing factors, and products. Water Res. 2016;90:15–23. https://doi.org/10.1016/j.watres.2015.11.068.

    Article  CAS  PubMed  Google Scholar 

  19. de la Casa-Resino I, Valdehita A, Soler F, Navas JM, Pérez-López M. Endocrine disruption caused by oral administration of atrazine in European quail (Coturnix coturnix coturnix). Comp Biochem Physiol C Toxicol Pharmacol. 2012;156(3–4):159–65. https://doi.org/10.1016/j.cbpc.2012.07.006.

    Article  CAS  PubMed  Google Scholar 

  20. Rohr JR, McCoy KA. A qualitative meta-analysis reveals consistent effects of atrazine on freshwater fish and amphibians. Environ Health Perspect. 2010;118(1):20–32. https://doi.org/10.1289/ehp.0901164.

    Article  CAS  PubMed  Google Scholar 

  21. Storrs SI, Kiesecker JM. Survivorship patterns of larval amphibians exposed to low concentrations of atrazine. Environ Health Perspect. 2004;112(10):1054–7. https://doi.org/10.1289/ehp.6821.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Hayes TB, Khoury V, Narayan A, Nazir M, Park A, Brown T, et al. Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis). Proc Natl Acad Sci U S A. 2010;107(10):4612–7. https://doi.org/10.1073/pnas.0909519107.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Sai L, Dong Z, Li L, Guo Q, Jia Q, Xie L, et al. Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine. Chemosphere. 2016;159:145–52. https://doi.org/10.1016/j.chemosphere.2016.05.008.

    Article  CAS  PubMed  Google Scholar 

  24. Meyer KD, Saletore Y, Zumbo P, Elemento O, Mason CE, Jaffrey SR. Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons. Cell. 2012;149(7):1635–46. https://doi.org/10.1016/j.cell.2012.05.003.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Sai L, Li Y, Zhang Y, Zhang J, Qu B, Guo Q, et al. Distinct m (6) a methylome profiles in poly(a) RNA from Xenopus laevis testis and that treated with atrazine. Chemosphere. 2020;245:125631. https://doi.org/10.1016/j.chemosphere.2019.125631.

    Article  CAS  PubMed  Google Scholar 

  26. Dominissini D, Moshitch-Moshkovitz S, Amariglio N, et al. Transcriptome-wide mapping of N6-Methyladenosine by m6A-Seq. Methods Enzymol. 2015;560:131–47. https://doi.org/10.1016/bs.mie.2015.03.001.

    Article  CAS  PubMed  Google Scholar 

  27. Tu Z, Wu L, Wang P, Hu Q, Tao C, Li K, et al. N6-Methylandenosine-related lncRNAs are potential biomarkers for predicting the overall survival of lower-grade Glioma patients. Front Cell Dev Biol. 2020;8:642. https://doi.org/10.3389/fcell.2020.00642.

    Article  PubMed  PubMed Central  Google Scholar 

  28. Auer PL, Doerge RW. Statistical design and analysis of RNA sequencing data. Genetics. 2010;185(2):405–16. https://doi.org/10.1534/genetics.110.114983.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Langmead B, Salzberg SL. Fast gapped-read alignment with bowtie 2. Nat Methods. 2012;9(4):357–9. https://doi.org/10.1038/nmeth.1923.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015;12(4):357–60. https://doi.org/10.1038/nmeth.3317.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Yu J, Chen M, Huang H, Zhu J, Song H, Zhu J, et al. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Res. 2018;46(3):1412–23. https://doi.org/10.1093/nar/gkx1182.

    Article  CAS  PubMed  Google Scholar 

  32. Huang X, Lv D, Yang X, Li M, Zhang H. m6A RNA methylation regulators could contribute to the occurrence of chronic obstructive pulmonary disease. J Cell Mol Med. 2020;24(21):12706–15. https://doi.org/10.1111/jcmm.15848.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Xiao S, Cao S, Huang Q, Xia L, Deng M, Yang M, et al. The RNA N (6)-methyladenosine modification landscape of human fetal tissues. Nat Cell Biol. 2019;21(5):651–61. https://doi.org/10.1038/s41556-019-0315-4.

    Article  CAS  PubMed  Google Scholar 

  34. Deng X, Chen K, Luo GZ, Weng X, Ji Q, Zhou T, et al. Widespread occurrence of N6-methyladenosine in bacterial mRNA. Nucleic Acids Res. 2015;43(13):6557–67. https://doi.org/10.1093/nar/gkv596.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Wu Y, Yang X, Chen Z, et al. m (6) A-induced lncRNA RP11 triggers the dissemination of colorectal cancer cells via upregulation of Zeb1. Mol Cancer. 2019;18(1):87.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Liu L, Lei X, Fang Z, Tang Y, Meng J, Wei Z. LITHOPHONE: improving lncRNA methylation site prediction using an ensemble predictor. Front Genet. 2020;11:545. https://doi.org/10.3389/fgene.2020.00545.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Zhu ZM, Huo FC, Pei DS. Function and evolution of RNA N6-methyladenosine modification. Int J Biol Sci. 2020;16(11):1929–40. https://doi.org/10.7150/ijbs.45231.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Tavera-Mendoza L, Ruby S, Brousseau P, Fournier M, Cyr D, Marcogliese D. Response of the amphibian tadpole (Xenopus laevis) to atrazine during sexual differentiation of the testis. Environ Toxicol Chem. 2002;21(3):527–31. https://doi.org/10.1002/etc.5620210309.

    Article  CAS  PubMed  Google Scholar 

  39. Orton F, Carr JA, Handy RD. Effects of nitrate and atrazine on larval development and sexual differentiation in the northern leopard frog Rana pipiens. Environ Toxicol Chem. 2006;25(1):65–71. https://doi.org/10.1897/05-136R.1.

    Article  CAS  PubMed  Google Scholar 

  40. Hanson ML, Solomon KR, Van Der Kraak GJ, et al. Effects of atrazine on fish, amphibians, and reptiles: update of the analysis based on quantitative weight of evidence. Crit Rev Toxicol. 2019;49(8):670–709. https://doi.org/10.1080/10408444.2019.1701985.

    Article  CAS  PubMed  Google Scholar 

  41. Lin Z, Hsu PJ, Xing X, Fang J, Lu Z, Zou Q, et al. Mettl3−/Mettl14-mediated mRNA N (6)-methyladenosine modulates murine spermatogenesis. Cell Res. 2017;27(10):1216–30. https://doi.org/10.1038/cr.2017.117.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Joshi M, Rajender S. Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility. Reprod Biol Endocrinol. 2020;18(1):103. https://doi.org/10.1186/s12958-020-00660-6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR. Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome. Nat Methods. 2015;12(8):767–72. https://doi.org/10.1038/nmeth.3453.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Wang X, Chen N, Du Z, et al. Bioinformatics analysis integrating metabolomics of m (6) a RNA microarray in intervertebral disc degeneration. Epigenomics. 2020;12(16):1419–41. https://doi.org/10.2217/epi-2020-0101.

    Article  CAS  PubMed  Google Scholar 

  45. Kasowitz SD, Ma J, Anderson SJ, Leu NA, Xu Y, Gregory BD, et al. Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and splicing during mouse oocyte development. PLoS Genet. 2018;14(5):e1007412. https://doi.org/10.1371/journal.pgen.1007412.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Lan T, Li H, Zhang D, Xu L, Liu H, Hao X, et al. KIAA1429 contributes to liver cancer progression through N6-methyladenosine-dependent post-transcriptional modification of GATA3. Mol Cancer. 2019;18(1):186. https://doi.org/10.1186/s12943-019-1106-z.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Esteller M. Non-coding RNAs in human disease. Nat Rev Genet. 2011;12(12):861–74. https://doi.org/10.1038/nrg3074.

    Article  CAS  PubMed  Google Scholar 

  48. Wang KC, Chang HY. Molecular mechanisms of long noncoding RNAs. Mol Cell. 2011;43(6):904–14. https://doi.org/10.1016/j.molcel.2011.08.018.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Yang D, Qiao J, Wang G, Lan Y, Li G, Guo X, et al. N6-Methyladenosine modification of lincRNA 1281 is critically required for mESC differentiation potential. Nucleic Acids Res. 2018;46(8):3906–20. https://doi.org/10.1093/nar/gky130.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Sai L, Qu B, Zhang J, Liu J, Jia Q, Bo C, et al. Analysis of long non-coding RNA involved in atrazine-induced testicular degeneration of Xenopus laevis. Environ Toxicol. 2019;34(4):505–12. https://doi.org/10.1002/tox.22704.

    Article  CAS  PubMed  Google Scholar 

  51. Ban Y, Tan P, Cai J, Li J, Hu M, Zhou Y, et al. LNCAROD is stabilized by m6A methylation and promotes cancer progression via forming a ternary complex with HSPA1A and YBX1 in head and neck squamous cell carcinoma. Mol Oncol. 2020;14(6):1282–96. https://doi.org/10.1002/1878-0261.12676.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Liu P, Zhang B, Chen Z, et al. m (6) A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway. Aging (Albany NY). 2020;12(6):5280–99.

    Article  CAS  Google Scholar 

  53. Bassham DC, Blatt MR. SNAREs: cogs and coordinators in signaling and development. Plant Physiol. 2008;147(4):1504–15. https://doi.org/10.1104/pp.108.121129.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Daste F, Galli T, Tareste D. Structure and function of longin SNAREs. J Cell Sci. 2015;128(23):4263–72. https://doi.org/10.1242/jcs.178574.

    Article  CAS  PubMed  Google Scholar 

  55. Campanoni P, Blatt MR. Membrane trafficking and polar growth in root hairs and pollen tubes. J Exp Bot. 2007;58(1):65–74. https://doi.org/10.1093/jxb/erl059.

    Article  CAS  PubMed  Google Scholar 

  56. Brahmaraju M, Shoeb M, Laloraya M, Kumar PG. Spatio-temporal organization of Vam6P and SNAP on mouse spermatozoa and their involvement in sperm-zona pellucida interactions. Biochem Biophys Res Commun. 2004;318(1):148–55. https://doi.org/10.1016/j.bbrc.2004.04.006.

    Article  CAS  PubMed  Google Scholar 

  57. Moreno RD, Ramalho-Santos J, Sutovsky P, et al. Vesicular traffic and golgi apparatus dynamics during mammalian spermatogenesis: implications for acrosome architecture. Biol Reprod. 2000;63(1):89–98. https://doi.org/10.1095/biolreprod63.1.89.

    Article  CAS  PubMed  Google Scholar 

  58. Ramalho-Santos J, Moreno RD, Wessel GM, Chan EKL, Schatten G. Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis. Exp Cell Res. 2001;267(1):45–60. https://doi.org/10.1006/excr.2000.5119.

    Article  CAS  PubMed  Google Scholar 

  59. Bedford L, Lowe J, Dick LR, Mayer RJ, Brownell JE. Ubiquitin-like protein conjugation and the ubiquitin-proteasome system as drug targets. Nat Rev Drug Discov. 2011;10(1):29–46. https://doi.org/10.1038/nrd3321.

    Article  CAS  PubMed  Google Scholar 

  60. Kerscher O, Felberbaum R, Hochstrasser M. Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol. 2006;22(1):159–80. https://doi.org/10.1146/annurev.cellbio.22.010605.093503.

    Article  CAS  PubMed  Google Scholar 

  61. Swatek KN, Komander D. Ubiquitin modifications. Cell Res. 2016;26(4):399–422. https://doi.org/10.1038/cr.2016.39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. McDowell GS, Philpott A. Ubiquitin-mediated proteolysis in Xenopus extract. Int J Dev Biol. 2016;60(7–8-9):263–70.

    Article  CAS  PubMed  Google Scholar 

  63. Freiman RN, Tjian R. Regulating the regulators: lysine modifications make their mark. Cell. 2003;112(1):11–7. https://doi.org/10.1016/S0092-8674(02)01278-3.

    Article  CAS  PubMed  Google Scholar 

  64. Sutovsky P. Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone. Microsc Res Tech. 2003;61(1):88–102. https://doi.org/10.1002/jemt.10319.

    Article  CAS  PubMed  Google Scholar 

  65. Halvorson LM. PACAP modulates GnRH signaling in gonadotropes. Mol Cell Endocrinol. 2014;385(1–2):45–55. https://doi.org/10.1016/j.mce.2013.09.029.

    Article  CAS  PubMed  Google Scholar 

  66. Ojeda SR, Lomniczi A. Puberty in 2013: Unravelling the mystery of puberty. Nat Rev Endocrinol. 2014;10(2):67–9. https://doi.org/10.1038/nrendo.2013.233.

    Article  PubMed  Google Scholar 

  67. Fagerstone KA, Miller LA, Killian G, et al. Review of issues concerning the use of reproductive inhibitors, with particular emphasis on resolving human-wildlife conflicts in North America. Integr Zool. 2010;5(1):15–30. https://doi.org/10.1111/j.1749-4877.2010.00185.x.

    Article  PubMed  Google Scholar 

  68. Suzuki H, Takemoto Y, Yamamoto T. Differential distribution of orexin-A-like and orexin receptor 1 (OX1R)-like immunoreactivities in the Xenopus pituitary. Tissue Cell. 2007;39(6):423–30. https://doi.org/10.1016/j.tice.2007.08.001.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Cloud-Seq Biotech Ltd. Co. (Shanghai, China) and Yuxing Medtech Ltd. Co. (Ji’nan, China) for supporting the high throughput sequencing instrument.

Funding

This work was supported by the Health Commission of Shandong Province (202012010629), Ji’nan Science and Technology Bureau (202019162), National Natural Science Foundation of China (81573198; 30901214), the Innovation Project of Shandong Academy of Medical Sciences, Academic Promotion Programme of Shandong First Medical University (2019QL001), Ministry of Science and Technology of PRC (2018ZX09711001–011).

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Author notes

  1. Xuejie Qi and Xiao Geng contributed equally to this work.

Authors and Affiliations

  1. Department of Toxicology, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, 18877 Jingshi Road, Lixia District, Ji’nan, Shandong, China

    Xuejie Qi, Xiao Geng, Juan Zhang, Xin Zhang, Qiang Jia, Wenhui Yin, Cunxiang Bo, Yan Liu, Linlin Sai, Mingming Han & Cheng Peng

  2. Shandong Medical College, Ji’nan, Shandong, China

    Binpeng Qu

  3. School of Laboratory Animal & Shandong Laboratory Animal Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan, Shandong, China

    Hao Li

  4. Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, Australia

    Cheng Peng

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Contributions

SL and HM conceived and supervised the study; ZJ and JQ designed experiments; YW, LH and BC conducted the experiments; QB LY and XZ prepared all figures and tables; QX wrote the main manuscript text; SL and PC andanalyzed the data and revised the manuscript. All authors reviewed the manuscript. The author(s) read and approved the final manuscript.

Corresponding authors

Correspondence to Linlin Sai or Mingming Han.

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The protocol was approved by the Committee on the Ethics of Animal Experiments of Shandong Academy of Occupational Health and Occupational Medicine.

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The authors declare that they have no competing interests.

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Supplementary Information

Additional file 1: Table S1.

The up and down methylated peaks.

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Qi, X., Geng, X., Zhang, J. et al. Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis. Genes and Environ 43, 49 (2021). https://doi.org/10.1186/s41021-021-00223-0

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Keywords

Genes and Environment

ISSN: 1880-7062

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