Articles

Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells

There is increasing evidence indicating an incidence of infertility and also the risk of endometrial cancers among smokers. However, the mechanism underlying nicotine adverse effect on female reproduction rema...

Authors: Fatemeh Zal, Amir Yarahmadi, Hamidreza Totonchi, Mahdi Barazesh and Mostafa Moradi Sarabi

Flow cytometric micronucleus assay and TGx-DDI transcriptomic biomarker analysis of ten genotoxic and non-genotoxic chemicals in human HepaRG™ cells

Modern testing paradigms seek to apply human-relevant cell culture models and integrate data from multiple test systems to accurately inform potential hazards and modes of action for chemical toxicology. In ge...

Authors: Julie K. Buick, Andrew Williams, Rémi Gagné, Carol D. Swartz, Leslie Recio, Stephen S. Ferguson and Carole L. Yauk

The prospective mathematical idea satisfying both radiation hormesis under low radiation doses and linear non-threshold theory under high radiation doses

It has yet to be determined whether or not the probability of developing cancer due to radiation exposure levels of low doses is proportional to the dose. Herein, for radiation hormesis occurring at low doses,...

Authors: Katsuhito Kino

Evaluation of acetone as a solvent for the Ames test

Acetone is a common alternative solvent used in the Ames test when test chemicals are unstable or poorly soluble in water or dimethyl sulfoxide (DMSO). Yet, there is a very limited number of studies evaluating...

Authors: Tomomi Shibata, Takeshi Yamagata, Akihiro Kawade, Shoji Asakura, Naoki Toritsuka, Naoki Koyama and Atsushi Hakura

Effects of acetaldehyde-induced DNA lesions on DNA metabolism

Acetaldehyde, produced upon exposure to alcohol, cigarette smoke, polluted air and sugar, is a highly reactive compound that is carcinogenic to humans and causes a variety of DNA lesions in living human cells....

Authors: Haruka Tsuruta, Yuina Sonohara, Kosuke Tohashi, Narumi Aoki Shioi, Shigenori Iwai and Isao Kuraoka

Black rain in Hiroshima: a critique to the Life Span Study of A-bomb survivors, basis of the linear no-threshold model

Ionizing radiation is regulated by the linear no-threshold model (LNT), which asserts that the lowest doses of ionizing radiation are hazardous in proportion to the dose and dose rate. LNT is based on the data...

Authors: Shizuyo Sutou

Assessing the endocrine disrupting potentials and genotoxicity in environmental samples from Taiwanese rivers

Surface waters receive a variety of organic pollutants via wastewater discharge, and sediment represents a sink for hydrophobic contaminants. In this study, we used in vitro yeast-based reporter gene assays an...

Authors: Pei-Hsin Chou, Chien-Hsun Chen, Kuang-Yu Chen, Fung-Chi Ko, Tsung-Ya Tsai and Yi-Po Yeh

Acid-specific formaldehyde donor is a potential, dual targeting cancer chemotherapeutic/chemo preventive drug for FANC/BRCA-mutant cancer

Development of chemotherapeutic/preventive drugs that selectively kill cancer - the Holy Grail of cancer research - is a major challenge. A particular difficulty arises when chemotherapeutics and radiation are...

Authors: John R. Ridpath and Jun Nakamura

Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

An extract from Spatholobus suberectus (S. suberectus) Dunn has been reported to show potent antimutagenic effects against N-alkyl-N-nitrosoureas in umu screening. The aim of this study was to identify the antimu...

Authors: Keiko Inami, Yoshihisa Asada, Takumi Harada, Yuta Okayama, Noriko Usui and Masataka Mochizuki

Development of a novel PIG-A gene mutation assay based on a GPI-anchored fluorescent protein sensor

Accumulation of somatic mutations caused by both endogenous and exogenous exposures is a high risk for human health, in particular, cancer. Efficient detection of somatic mutations is crucial for risk assessme...

Authors: Xu Tian, Youjun Chen and Jun Nakamura

Diurnal variation of salivary oxidative stress marker 8-hydroxyguanine

Oxidative stress is a risk factor for life-style related diseases, including cancer. We recently reported that the oxidative stress marker 8-hydroxyguanine (8-OHGua) can be measured in saliva non-invasively. U...

Authors: Sintaroo Watanabe, Yuya Kawasaki and Kazuaki Kawai

Association between ACTN3 and acute mountain sickness

During the process of acclimatization, when our organism needs to adjust several metabolic processes in the attempt of establishing a better oxygenation, it is normal that individuals present some symptoms tha...

Authors: Ricardo Muller Bottura, Giscard Humberto Oliveira Lima, Debora Cristina Hipolide and João Bosco Pesquero

The 32nd summer school of the Research Community for Mechanisms of Mutations

The 32nd summer school of the Research Community for Mechanisms of Mutations was held at Inter-University Seminar House in Hachioji city, Tokyo, from September 7 to 8, 2019. Thirty-eight people attended this a...

Authors: Masanobu Kawanishi

Comprehensive framework between environment and genomic stability: the open symposium of the Japanese Environmental Mutagen Society (JEMS) in 2019

The open symposium of the Japanese Environmental Mutagen Society (JEMS), under the title of “Comprehensive framework between environment and genomic stability,” was held in the Main Conference Room of the Foun...

Authors: Katsuyoshi Horibata, Masashi Sekimoto and Kei-ichi Sugiyama

Effect of MMP-2 gene silencing on radiation-induced DNA damage in human normal dermal fibroblasts and breast cancer cells

Diagnostic and therapeutic ionizing radiation (IR) is one of the well known long term risk factors of breast cancer. Extremely lethal consequences of IR causes double-strand breaks, which are mainly responsibl...

Authors: Gugalavath Shailender, Seema Kumari, Patnala Kiranmayi and Rama Rao Malla

Comparative study of cytotoxic effects induced by environmental genotoxins using XPC- and CSB-deficient human lymphoblastoid TK6 cells

The human genome is constantly exposed to numerous environmental genotoxicants. To prevent the detrimental consequences induced by the expansion of damaged cells, cellular protective systems such as nucleotide...

Authors: Akira Sassa, Takayuki Fukuda, Akiko Ukai, Maki Nakamura, Michihito Takabe, Takeji Takamura-Enya, Masamitsu Honma and Manabu Yasui

Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy

Frustrated phagocytosis occurs when an asbestos fiber > 10 μm in length is engulfed imperfectly by a macrophage, and it is believed to be associated with chromosomal instability. Few studies have focused on dy...

Authors: Takenori Ishida, Nobutoshi Fujihara, Tomoki Nishimura, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda and Akio Kuroda

Evaluation of the novel liver micronucleus assay using formalin-fixed tissues

The repeated-dose liver micronucleus (RDLMN) assay is an effective and important in vivo test for detecting genotoxic compounds, particularly for those that require metabolic activation to show genotoxicity. I...

Authors: Shuichi Hamada, Miyuki Shigano, Satoru Kawakami, Maya Ueda, Hajime Sui, Katsuya Yamada, Soichiro Hagio, Ayaka Momonami, Akihisa Maeda, Yukari Terashima, Wakako Ohyama, Takeshi Morita and Makoto Hayashi

Purification and interactions of the MucA’ and MucB proteins constituting the DNA polymerase RI

The MucA’ and MucB proteins comprise the core of DNA polymerase RI which is a strong mutator utilized in mutagenicity assays such as the standard Ames test. A close relative DNA polymerase V, composed of the h...

Authors: Petr Grúz, Kei-ichi Sugiyama, Masamitsu Honma and Takehiko Nohmi

Correction to: Low-dose radiation from A-bombs elongated lifespan and reduced cancer mortality relative to un-irradiated individuals

In the original publication of this article [1], the author pointed out the reference list mismatched citations in the main text. All citations and reference has been updated.

Authors: Shizuyo Sutou

Chloroethylating anticancer drug-induced mutagenesis and its repair in Escherichia coli

Chloroethylnitrosourea (CENU) derivatives, such as nimustine (ACNU) and carmustine (BCNU), are employed in brain tumor chemotherapy due to their ability to cross the blood-brain barrier. They are thought to su...

Authors: Yoko Yamada, Shinji Watanabe, Keinosuke Okamoto, Sakae Arimoto, Eizo Takahashi, Kazuo Negishi and Tomoe Negishi

Association of ACE gene polymorphism with cardiovascular determinants of trained and untrained Iranian men

The insertion (I) rather than deletion (D) of human angiotensin converting enzyme gene (ACE) is associated with lower circulating ACE activity and with endurance performance among Caucasians. The frequency of ...

Authors: Akram Falahati and Hamid Arazi

Development of novel and safer anti-breast cancer agents, SS1020 and SS5020, based on a fundamental carcinogenic research

Tamoxifen (TAM) has been prescribed worldwide to patients with and women at high-risk of breast cancer. However, long-term use of TAM increases the incidence of endometrial cancer. The carcinogenic mechanisms ...

Authors: Yoshinori Okamoto and Shinya Shibutani

Importance of finding the bona fide target of the Fanconi anemia pathway

Fanconi anemia (FA) is a rare genetic disease characterized by the deficiency of the cellular response and repair pathway for DNA interstrand crosslink (ICL) damage. Although recent studies have revealed the d...

Authors: Wataru Sakai and Kaoru Sugasawa

Evaluation of the genotoxicity of PM2.5 collected by a high-volume air sampler with impactor

The harmful effects of fine particles with an aerodynamic diameter less than 2.5 μm (PM2.5) on respiratory organs are emphasized in pollution studies because PM2.5 have high deposition rates in the respiratory...

Authors: Kazutoshi Sugita, Yuka Kin, Mayuko Yagishita, Fumikazu Ikemori, Kimiyo Kumagai, Toshihiko Ohara, Makoto Kinoshita, Kazuyuki Nishimura, Yukihiko Takagi and Daisuke Nakajima

Standard protocol for the total red blood cell Pig-a assay used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society

The Pig-a assay, a promising tool for evaluating in vivo genotoxicity, is based on flow cytometric enumeration of red blood cells (RBCs) that are deficient in glycosylphosphatidylinositol anchor protein. Various ...

Authors: Satsuki Chikura, Takafumi Kimoto, Satoru Itoh, Hisakazu Sanada, Shigeharu Muto and Katsuyoshi Horibata

High-content imaging analyses of γH2AX-foci and micronuclei in TK6 cells elucidated genotoxicity of chemicals and their clastogenic/aneugenic mode of action

The in vitro micronucleus (MN) test is an important component of a genotoxicity test battery that evaluates chemicals. Although the standard method of manually scoring micronucleated (MNed) cells by microscope...

Authors: Akira Takeiri, Kaori Matsuzaki, Shigeki Motoyama, Mariko Yano, Asako Harada, Chiaki Katoh, Kenji Tanaka and Masayuki Mishima

Current perspectives on mechanisms of ribonucleotide incorporation and processing in mammalian DNA

Ribonucleotides, which are RNA precursors, are often incorporated into DNA during replication. Although embedded ribonucleotides in the genome are efficiently removed by canonical ribonucleotide excision repai...

Authors: Akira Sassa, Manabu Yasui and Masamitsu Honma

Mechanism and regulation of DNA damage recognition in nucleotide excision repair

Nucleotide excision repair (NER) is a versatile DNA repair pathway, which can remove an extremely broad range of base lesions from the genome. In mammalian global genomic NER, the XPC protein complex initiates...

Authors: Masayuki Kusakabe, Yuki Onishi, Haruto Tada, Fumika Kurihara, Kanako Kusao, Mari Furukawa, Shigenori Iwai, Masayuki Yokoi, Wataru Sakai and Kaoru Sugasawa

Inhibition of NF-kappaB transcriptional activity enhances fucoxanthinol-induced apoptosis in colorectal cancer cells

Evidence from epidemiological and experimental studies has shown that the etiology of colorectal cancer (CRC) is related to lifestyle, mainly diet. At the same time, there are many foods and beverages that hav...

Authors: Shuya Tamura, Takumi Narita, Gen Fujii, Shingo Miyamoto, Takahiro Hamoya, Yurie Kurokawa, Maiko Takahashi, Kouhei Miki, Yui Matsuzawa, Masami Komiya, Masaru Terasaki, Tomohiro Yano and Michihiro Mutoh

Summarized data of genotoxicity tests for designated food additives in Japan

The Ministry of Health, Labour and Welfare has carried out genotoxicity tests for food additives used in Japan in cooperation with the Japan Food Additives Association since 1979. Hayashi et al. summarized the...

Authors: Masami Yamada and Masamitsu Honma

Low-dose radiation from A-bombs elongated lifespan and reduced cancer mortality relative to un-irradiated individuals

The US National Academy of Sciences (NAS) presented the linear no-threshold hypothesis (LNT) in 1956, which indicates that the lowest doses of ionizing radiation are hazardous in proportion to the dose. This s...

Authors: Shizuyo Sutou

Change over time of the mutagenicity in the lungs of gpt delta transgenic mice by extract of airborne particles collected from ambient air in the Tokyo metropolitan area

Previously we found that DNA adducts were accumulated in the lungs of the rats exposed to ambient air in the Tokyo metropolitan area. To examine chronological change in in vivo mutagenicity of airborne particl...

Authors: Yasunobu Aoki, Daisuke Nakajima, Michiyo Matsumoto, Mayuko Yagishita, Michi Matsumoto, Rie Yanagisawa, Sumio Goto, Kenichi Masumura and Takehiko Nohmi

Oligo swapping method for in vitro DNA repair substrate containing a single DNA lesion at a specific site

A wide variety of DNA lesions interfere with replication and transcription, leading to mutations and cell death. DNA repair mechanisms act upon these DNA lesions present in the genomic DNA. To investigate a DN...

Authors: Mika Yukutake, Mika Hayashida, Narumi Shioi Aoki and Isao Kuraoka

Challenges of young scientists at the cutting-edge of genotoxicity research: the open symposium of the Japanese Environmental Mutagen Society (JEMS), 2018

The Open Symposium of the Japanese Environmental Mutagen Society (JEMS) entitled “Challenges of Young Scientists at the Cutting-edge of Genotoxicity Research” was held in the Main Conference Room of the Founda...

Authors: Manabu Yasui, Shigeharu Muto and Akira Sassa

Analysis of large deletion mutations induced by abasic site analog in human cells

Abasic sites are formed spontaneously and by nucleobase chemical modifications and base excision repair. A chemically stable abasic site analog was site-specifically introduced into replicable plasmid DNAs, wh...

Authors: Tetsuya Suzuki, Yuri Katayama, Yasuo Komatsu and Hiroyuki Kamiya

Chromium (VI) – induced stress response in the plant Plantago ovata Forsk in vitro

Plants experience severe physiological stress from heavy metal pollution caused by improper discarding of the industrial wastes. Hexavalent chromium [Cr (VI)] is one of the major heavy metal pollutants in Indi...

Authors: Debangana Kundu, Sankalan Dey and Sarmistha Sen Raychaudhuri

Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests

According to the linear no-threshold model (LNT), even the smallest amount of radiation is hazardous. Although the LNT is not based on solid data, this hypothesis has been applied to mutagens and carcinogens. ...

Authors: Shizuyo Sutou, Akiko Koeda, Kana Komatsu, Toshiyuki Shiragiku, Hiroshi Seki, Kohji Yamakage, Takeru Niitsuma, Toshiyuki Kudo and Akihiro Wakata

Meeting report, ICH M7 relevant workshop: use of (Q)SAR systems and expert judgment

Use of Quantitative Structure-Activity Relationships ((Q)SAR) prediction tools has been increasing since the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use ...

Authors: Masayuki Mishima, Tsuneo Hashizume, Yu Haranosono, Yusuke Nagato, Kentaro Takeshita, Junichi Fukuchi and Masamitsu Homma

Comparison of structures among Saccharomyces cerevisiae Grxs proteins

Glutaredoxins (Grxs) comprise a group of glutathione (GSH)-dependent oxidoreductase enzymes that respond to oxidative stress and sustain redox homeostasis. Saccharomyces cerevisiae Grx has a similar interaction p...

Authors: Mohnad Abdalla, Wafa Ali Eltayb and Aadil Yousif

Mutagenic properties of dimethylaniline isomers in mice as evaluated by comet, micronucleus and transgenic mutation assays

The carcinogenic potential of dimethylaniline (DMA) isomers in rodents and humans has been previously reported, and there is sufficient evidence for the carcinogenicity of 2,6-DMA in experimental animals. The ...

Authors: Arihiro Kohara, Mariko Matsumoto, Akihiko Hirose, Makoto Hayashi, Masamitsu Honma and Takayoshi Suzuki

Structure analysis of yeast glutaredoxin Grx6 protein produced in Escherichia coli

Grx6 is a yeast Golgi/endoplasmic reticulum protein involved in iron-sulfur binding that belongs to monothiol glutaredoxin-protein family. Grx6 has been biochemically characterized previously. Grx6 contains a ...

Authors: Mohnad Abdalla, Wafa Ali Eltayb, Amr Ahmed El-Arabey, Raihan Mo, T. I. M. Dafaalla, Hamed I. Hamouda, Eijaz Ahmed Bhat, Annoor Awadasseid and Hassan Abdellha Ahmed Ali

Simultaneous and absolute quantification of nucleoside triphosphates using liquid chromatography–triple quadrupole tandem mass spectrometry

Nucleoside triphosphates participate in fundamental cellular processes as building blocks of DNA and RNA, energy carriers, and cofactors in enzymatic reactions, and their balance is tightly regulated. Here, we...

Authors: Shun Matsuda and Toshihiko Kasahara

Correction to: New insight for metformin against bladder cancer

The author noted a few errors in the original article [1] need correction. The correct content is below.

Authors: Amr Ahmed EL-Arabey

Ozone augments interleukin-8 production induced by ambient particulate matter

Experimental and controlled human exposure studies have demonstrated additive effects of ambient particulate matter and ozone on health. A few epidemiological studies have suggested that ambient particulate ma...

Authors: Jun Kurai, Kunishige Onuma, Hiroyuki Sano, Futoshi Okada and Masanari Watanabe

Does the prenatal bisphenol A exposure alter DNA methylation levels in the mouse hippocampus?: An analysis using a high-sensitivity methylome technique

There is still considerable debate about the effects of exposure to bisphenol A (BPA) an endocrine disrupter at low doses. Recently, many studies using animal models have shown that prenatal BPA exposure induc...

Authors: Toshiki Aiba, Toshiyuki Saito, Akiko Hayashi, Shinji Sato, Harunobu Yunokawa, Toru Maruyama, Wataru Fujibuchi and Seiichiroh Ohsako

Greetings from the new regime

Authors: Masami Yamada

Advantages of evaluating γH2AX induction in non-clinical drug development

γH2AX, the phosphorylated form of a histone variant H2AX at Ser 139, is already widely used as a biomarker to research the fundamental biology of DNA damage and repair and to assess the risk of environmental c...

Authors: Shigeki Motoyama, Akira Takeiri, Kenji Tanaka, Asako Harada, Kaori Matsuzaki, Junko Taketo, Saori Matsuo, Etsuko Fujii and Masayuki Mishima

The general Assembly of the International Association of Environmental Mutagenesis and Genomics Societies (IAEMGS) in 2017

The International Association of Environmental Mutagenesis and Genomics Societies (IAEMGS) is a global organization that promotes applied and basic research on environmental mutagenesis and genomics. IAEMGS is...

Authors: Takehiko Nohmi, Catarina S. Takahashi, Byung-Mu Lee, Michael F. Fenech and Masami Yamada

Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD

Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as...

Authors: Kazuaki Kawai, Hiroshi Kasai, Yun-Shan Li, Yuya Kawasaki, Shintaro Watanabe, Masanori Ohta, Toru Honda and Hiroshi Yamato